Dietary methanol regulates human gene activity.
Methanol (MeOH) is considered to be a poison in humans because of the alcohol dehydrogenase (ADH)-mediated conversion of MeOH to formaldehyde (FA), which is toxic. Our recent genome-wide analysis of the mouse brain demonstrated that an increase in endogenous MeOH after ADH inhibition led to a signif...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4102594?pdf=render |
| _version_ | 1818320430937669632 |
|---|---|
| author | Anastasia V Shindyapina Igor V Petrunia Tatiana V Komarova Ekaterina V Sheshukova Vyacheslav S Kosorukov Gleb I Kiryanov Yuri L Dorokhov |
| author_facet | Anastasia V Shindyapina Igor V Petrunia Tatiana V Komarova Ekaterina V Sheshukova Vyacheslav S Kosorukov Gleb I Kiryanov Yuri L Dorokhov |
| author_sort | Anastasia V Shindyapina |
| collection | DOAJ |
| description | Methanol (MeOH) is considered to be a poison in humans because of the alcohol dehydrogenase (ADH)-mediated conversion of MeOH to formaldehyde (FA), which is toxic. Our recent genome-wide analysis of the mouse brain demonstrated that an increase in endogenous MeOH after ADH inhibition led to a significant increase in the plasma MeOH concentration and a modification of mRNA synthesis. These findings suggest endogenous MeOH involvement in homeostasis regulation by controlling mRNA levels. Here, we demonstrate directly that study volunteers displayed increasing concentrations of MeOH and FA in their blood plasma when consuming citrus pectin, ethanol and red wine. A microarray analysis of white blood cells (WBC) from volunteers after pectin intake showed various responses for 30 significantly differentially regulated mRNAs, most of which were somehow involved in the pathogenesis of Alzheimer's disease (AD). There was also a decreased synthesis of hemoglobin mRNA, HBA and HBB, the presence of which in WBC RNA was not a result of red blood cells contamination because erythrocyte-specific marker genes were not significantly expressed. A qRT-PCR analysis of volunteer WBCs after pectin and red wine intake confirmed the complicated relationship between the plasma MeOH content and the mRNA accumulation of both genes that were previously identified, namely, GAPDH and SNX27, and genes revealed in this study, including MME, SORL1, DDIT4, HBA and HBB. We hypothesized that human plasma MeOH has an impact on the WBC mRNA levels of genes involved in cell signaling. |
| first_indexed | 2024-12-13T10:24:53Z |
| format | Article |
| id | doaj.art-d7166723fba340188a38089c1a52deae |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T10:24:53Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d7166723fba340188a38089c1a52deae2022-12-21T23:51:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0197e10283710.1371/journal.pone.0102837Dietary methanol regulates human gene activity.Anastasia V ShindyapinaIgor V PetruniaTatiana V KomarovaEkaterina V SheshukovaVyacheslav S KosorukovGleb I KiryanovYuri L DorokhovMethanol (MeOH) is considered to be a poison in humans because of the alcohol dehydrogenase (ADH)-mediated conversion of MeOH to formaldehyde (FA), which is toxic. Our recent genome-wide analysis of the mouse brain demonstrated that an increase in endogenous MeOH after ADH inhibition led to a significant increase in the plasma MeOH concentration and a modification of mRNA synthesis. These findings suggest endogenous MeOH involvement in homeostasis regulation by controlling mRNA levels. Here, we demonstrate directly that study volunteers displayed increasing concentrations of MeOH and FA in their blood plasma when consuming citrus pectin, ethanol and red wine. A microarray analysis of white blood cells (WBC) from volunteers after pectin intake showed various responses for 30 significantly differentially regulated mRNAs, most of which were somehow involved in the pathogenesis of Alzheimer's disease (AD). There was also a decreased synthesis of hemoglobin mRNA, HBA and HBB, the presence of which in WBC RNA was not a result of red blood cells contamination because erythrocyte-specific marker genes were not significantly expressed. A qRT-PCR analysis of volunteer WBCs after pectin and red wine intake confirmed the complicated relationship between the plasma MeOH content and the mRNA accumulation of both genes that were previously identified, namely, GAPDH and SNX27, and genes revealed in this study, including MME, SORL1, DDIT4, HBA and HBB. We hypothesized that human plasma MeOH has an impact on the WBC mRNA levels of genes involved in cell signaling.http://europepmc.org/articles/PMC4102594?pdf=render |
| spellingShingle | Anastasia V Shindyapina Igor V Petrunia Tatiana V Komarova Ekaterina V Sheshukova Vyacheslav S Kosorukov Gleb I Kiryanov Yuri L Dorokhov Dietary methanol regulates human gene activity. PLoS ONE |
| title | Dietary methanol regulates human gene activity. |
| title_full | Dietary methanol regulates human gene activity. |
| title_fullStr | Dietary methanol regulates human gene activity. |
| title_full_unstemmed | Dietary methanol regulates human gene activity. |
| title_short | Dietary methanol regulates human gene activity. |
| title_sort | dietary methanol regulates human gene activity |
| url | http://europepmc.org/articles/PMC4102594?pdf=render |
| work_keys_str_mv | AT anastasiavshindyapina dietarymethanolregulateshumangeneactivity AT igorvpetrunia dietarymethanolregulateshumangeneactivity AT tatianavkomarova dietarymethanolregulateshumangeneactivity AT ekaterinavsheshukova dietarymethanolregulateshumangeneactivity AT vyacheslavskosorukov dietarymethanolregulateshumangeneactivity AT glebikiryanov dietarymethanolregulateshumangeneactivity AT yurildorokhov dietarymethanolregulateshumangeneactivity |