Isolation and Identification of Protease Enzyme Producing Bacteria from Fermentation of Gonad Sea Urchin (Echinothrix calamaris)
Bekasang of gonad sea urchin is one of the traditional fermentation products which generally involves microorganism spontaneous fermentation. Fermented paste products have a long shelf life and are processed quite easily using protease enzymes. Good exploration of producing protease from bakasang is...
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| Format: | Article |
| Language: | English |
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Diponegoro University; Association of Indonesian Coastal Management Experts
2019-01-01
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| Series: | Ilmu Kelautan |
| Subjects: | |
| Online Access: | https://ejournal.undip.ac.id/index.php/ijms/article/view/15084 |
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| author | Siani La Jamaludin Johanis Fritzgal Rehena Cecilia Anna Seumahu Dominggus Rumahlatu |
| author_facet | Siani La Jamaludin Johanis Fritzgal Rehena Cecilia Anna Seumahu Dominggus Rumahlatu |
| author_sort | Siani La Jamaludin |
| collection | DOAJ |
| description | Bekasang of gonad sea urchin is one of the traditional fermentation products which generally involves microorganism spontaneous fermentation. Fermented paste products have a long shelf life and are processed quite easily using protease enzymes. Good exploration of producing protease from bakasang is needed to obtain the protease enzyme-producing microorganism with different characters. The method used in this research is screening with clear zone, measuring the activity of crude extract of protease enzyme characterization of bacteria through gram staining. Identification of potential microorganisms through 16S rRNA sequence. The results showed that there were eight isolates of protease enzyme-producing bacteria (G1, G2, G3, G4, G5, G6, G7, and G8) indicated by clear zones around single-colonic bacterial streaks. Only five bacterial isolates (G1, G4, G6, G7, and G8) were tested for the enzyme activity. These isolates have characteristics of positive gram bacteria. The interpretation of the results of molecular analysis using PCR and BLASTN sequences of 16S rRNA gene from five bacterial isolates, showed the identity of bacteria as: G1 was Staphylococcus piscifermentans strain CIP103958 with 99% similarity; Isolate G4 was Staphylococcus saprophyticus strain ATCC 15305 with 99% similarity; Isolate G6 was Staphylococcus condimenti F-2 strain with 99% similarity; Isolate G7 was Bacillus amyloliquefaciens subsp. plantarum strain FZB42 with 99% similarity; And G8 isolates was Lactobacillus plantarum strain JCM 1149 with 99% similarity. |
| first_indexed | 2024-04-14T05:13:59Z |
| format | Article |
| id | doaj.art-d723896b4e624b2298a4e5c253e8d46c |
| institution | Directory Open Access Journal |
| issn | 0853-7291 2406-7598 |
| language | English |
| last_indexed | 2024-04-14T05:13:59Z |
| publishDate | 2019-01-01 |
| publisher | Diponegoro University; Association of Indonesian Coastal Management Experts |
| record_format | Article |
| series | Ilmu Kelautan |
| spelling | doaj.art-d723896b4e624b2298a4e5c253e8d46c2022-12-22T02:10:26ZengDiponegoro University; Association of Indonesian Coastal Management ExpertsIlmu Kelautan0853-72912406-75982019-01-0123418719810.14710/ik.ijms.23.4.187-19814369Isolation and Identification of Protease Enzyme Producing Bacteria from Fermentation of Gonad Sea Urchin (Echinothrix calamaris)Siani La Jamaludin0Johanis Fritzgal Rehena1Cecilia Anna Seumahu2Dominggus Rumahlatu3Postgraduate Biology Education Study Program, Pattimura UniversityBiology Education Study Program, Faculty of Teacher Training and Education Science, Pattimura UniversityBiology Department, Faculty Mathematics and Science, Pattimura UniversityBiology Education Study Program, Faculty of Teacher Training and Education Science, Pattimura UniversityBekasang of gonad sea urchin is one of the traditional fermentation products which generally involves microorganism spontaneous fermentation. Fermented paste products have a long shelf life and are processed quite easily using protease enzymes. Good exploration of producing protease from bakasang is needed to obtain the protease enzyme-producing microorganism with different characters. The method used in this research is screening with clear zone, measuring the activity of crude extract of protease enzyme characterization of bacteria through gram staining. Identification of potential microorganisms through 16S rRNA sequence. The results showed that there were eight isolates of protease enzyme-producing bacteria (G1, G2, G3, G4, G5, G6, G7, and G8) indicated by clear zones around single-colonic bacterial streaks. Only five bacterial isolates (G1, G4, G6, G7, and G8) were tested for the enzyme activity. These isolates have characteristics of positive gram bacteria. The interpretation of the results of molecular analysis using PCR and BLASTN sequences of 16S rRNA gene from five bacterial isolates, showed the identity of bacteria as: G1 was Staphylococcus piscifermentans strain CIP103958 with 99% similarity; Isolate G4 was Staphylococcus saprophyticus strain ATCC 15305 with 99% similarity; Isolate G6 was Staphylococcus condimenti F-2 strain with 99% similarity; Isolate G7 was Bacillus amyloliquefaciens subsp. plantarum strain FZB42 with 99% similarity; And G8 isolates was Lactobacillus plantarum strain JCM 1149 with 99% similarity.https://ejournal.undip.ac.id/index.php/ijms/article/view/15084BekasangProteaseGonad Sea Urchins |
| spellingShingle | Siani La Jamaludin Johanis Fritzgal Rehena Cecilia Anna Seumahu Dominggus Rumahlatu Isolation and Identification of Protease Enzyme Producing Bacteria from Fermentation of Gonad Sea Urchin (Echinothrix calamaris) Ilmu Kelautan Bekasang Protease Gonad Sea Urchins |
| title | Isolation and Identification of Protease Enzyme Producing Bacteria from Fermentation of Gonad Sea Urchin (Echinothrix calamaris) |
| title_full | Isolation and Identification of Protease Enzyme Producing Bacteria from Fermentation of Gonad Sea Urchin (Echinothrix calamaris) |
| title_fullStr | Isolation and Identification of Protease Enzyme Producing Bacteria from Fermentation of Gonad Sea Urchin (Echinothrix calamaris) |
| title_full_unstemmed | Isolation and Identification of Protease Enzyme Producing Bacteria from Fermentation of Gonad Sea Urchin (Echinothrix calamaris) |
| title_short | Isolation and Identification of Protease Enzyme Producing Bacteria from Fermentation of Gonad Sea Urchin (Echinothrix calamaris) |
| title_sort | isolation and identification of protease enzyme producing bacteria from fermentation of gonad sea urchin echinothrix calamaris |
| topic | Bekasang Protease Gonad Sea Urchins |
| url | https://ejournal.undip.ac.id/index.php/ijms/article/view/15084 |
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