Selection and evaluation of reference genes for expression analysis using qRT-PCR in the beet armyworm Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae).

Selection and evaluation of reference genes for expression analysis using qRT-PCR in the beet armyworm Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae).

Quantitative real-time PCR (qRT-PCR) is a reliable and reproducible technique for measuring and evaluating changes in gene expression. The most common method for analyzing qRT-PCR data is to normalize mRNA levels of target genes to internal reference genes. Evaluating and selecting stable reference...

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Bibliographic Details
Main Authors: Xun Zhu, Miao Yuan, Muhammad Shakeel, Youjun Zhang, Shaoli Wang, Xin Wang, Sha Zhan, Tinghao Kang, Jianhong Li
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24454743/?tool=EBI

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https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24454743/?tool=EBI

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