Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping Events
The trap stiffness us the key property in using optical tweezers as a force transducer. Force reconstruction via maximum-likelihood-estimator analysis (FORMA) determines the optical trap stiffness based on estimation of the particle velocity from statistical trajectories. Using a modification of thi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/5/570 |
| _version_ | 1797533777715527680 |
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| author | Alexander B. Stilgoe Declan J. Armstrong Halina Rubinsztein-Dunlop |
| author_facet | Alexander B. Stilgoe Declan J. Armstrong Halina Rubinsztein-Dunlop |
| author_sort | Alexander B. Stilgoe |
| collection | DOAJ |
| description | The trap stiffness us the key property in using optical tweezers as a force transducer. Force reconstruction via maximum-likelihood-estimator analysis (FORMA) determines the optical trap stiffness based on estimation of the particle velocity from statistical trajectories. Using a modification of this technique, we determine the trap stiffness for a two micron particle within 2 ms to a precision of ∼10% using camera measurements at 10 kfps with the contribution of pixel noise to the signal being larger the level Brownian motion. This is done by observing a particle fall into an optical trap once at a high stiffness. This type of calibration is attractive, as it avoids the use of a nanopositioning stage, which makes it ideal for systems of large numbers of particles, e.g., micro-fluidics or active matter systems. |
| first_indexed | 2024-03-10T11:20:26Z |
| format | Article |
| id | doaj.art-d7398d4dfa4d481aaaeb5354f74b1fab |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T11:20:26Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-d7398d4dfa4d481aaaeb5354f74b1fab2023-11-21T20:07:12ZengMDPI AGMicromachines2072-666X2021-05-0112557010.3390/mi12050570Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping EventsAlexander B. Stilgoe0Declan J. Armstrong1Halina Rubinsztein-Dunlop2School of Mathematics and Physics, The University of Queensland, St. Lucia, Brisbane, QLD 4072, AustraliaSchool of Mathematics and Physics, The University of Queensland, St. Lucia, Brisbane, QLD 4072, AustraliaSchool of Mathematics and Physics, The University of Queensland, St. Lucia, Brisbane, QLD 4072, AustraliaThe trap stiffness us the key property in using optical tweezers as a force transducer. Force reconstruction via maximum-likelihood-estimator analysis (FORMA) determines the optical trap stiffness based on estimation of the particle velocity from statistical trajectories. Using a modification of this technique, we determine the trap stiffness for a two micron particle within 2 ms to a precision of ∼10% using camera measurements at 10 kfps with the contribution of pixel noise to the signal being larger the level Brownian motion. This is done by observing a particle fall into an optical trap once at a high stiffness. This type of calibration is attractive, as it avoids the use of a nanopositioning stage, which makes it ideal for systems of large numbers of particles, e.g., micro-fluidics or active matter systems.https://www.mdpi.com/2072-666X/12/5/570optical tweezersoptically driven swimmersswimmersswimming cellsmanipulationcalibration |
| spellingShingle | Alexander B. Stilgoe Declan J. Armstrong Halina Rubinsztein-Dunlop Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping Events Micromachines optical tweezers optically driven swimmers swimmers swimming cells manipulation calibration |
| title | Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping Events |
| title_full | Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping Events |
| title_fullStr | Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping Events |
| title_full_unstemmed | Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping Events |
| title_short | Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping Events |
| title_sort | enhanced signal to noise and fast calibration of optical tweezers using single trapping events |
| topic | optical tweezers optically driven swimmers swimmers swimming cells manipulation calibration |
| url | https://www.mdpi.com/2072-666X/12/5/570 |
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