Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana

Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana

Abstract Background To date, CRISPR/Cas9 RNP editing tools have not been applied to the genetic modification of banana. Here, the establishment of a PEG-mediated banana protoplast transformation system makes it possible to build an efficient DNA-free method for a site-directed mutagenesis system. Re...

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Bibliographic Details
Main Authors: Shaoping Wu, Haocheng Zhu, Jinxing Liu, Qiaosong Yang, Xiuhong Shao, Fangcheng Bi, Chunhua Hu, Heqiang Huo, Kunling Chen, Ganjun Yi
Format: Article
Language:English
Published: BMC 2020-09-01
Series:BMC Plant Biology
Subjects:
PEG-mediated
Protoplast transformation
Deep amplicon sequencing
Genome editing
DNA-free
Online Access:http://link.springer.com/article/10.1186/s12870-020-02609-8

Internet

http://link.springer.com/article/10.1186/s12870-020-02609-8

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