An optimized protocol for detecting guard cell specific gene expression by in situ RT-PCR in Brassica rapa

An optimized protocol for detecting guard cell specific gene expression by in situ RT-PCR in Brassica rapa

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It is important to detect specific genes expressed in the guard cells, which control gas exchange and play key roles in response to drought and salt stresses. Due to the genetic transformation of Chinese cabbage (Brassica rapa) has not been well developed, in situ RT-PCR is a valuable option for det...

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Bibliographic Details
Main Authors: Yingying Song, Xinlei Guo, Jian Wu, Jianli Liang, Runmao Lin, Zifu Yan, Xiaowu Wang
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2022-05-01
Series:Horticultural Plant Journal
Subjects:
Brassica rapa
In situ RT-PCR
Gene expression
Bra001929
Protocol
Online Access:http://www.sciencedirect.com/science/article/pii/S2468014121001412

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http://www.sciencedirect.com/science/article/pii/S2468014121001412

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