Intra specific DNA sequence polymorphism and genetic markers in the mitochon drial COII gene from five color patterns of Oujiang color carp(5种体色瓯江彩鲤线粒体COII基因的序列差异和遗传标记研究)

利用PCR方法扩增获得瓯江彩鲤(Cyprinus carpio var. color)线粒体DNA的COII基因,测定该基因片段序列.分析了瓯江彩鲤“全红”、“粉玉”、“粉花”、“麻花”和“大花”共20只个体的序列核苷酸位点差异和遗传多态性.结果表明,“大花”和“全红”遗传多样性最为丰富,“粉花”的遗传多样性相对贫乏.在长度为604 bp的基因片段中,共检测到9个多态性核苷酸位点(占1.49%) ,20只个体具有4种基因型,5种体色各自的平均核苷酸位点差异分别为0.38%、0.2%、0、0.13%和0.47%. UPGMA分子系统聚类树显示,“粉花”、“麻花”和“粉玉”的遗传关系接近;“全红”...

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Bibliographic Details
Main Authors: LVYao-ping(吕耀平), HUZe-hui(胡则辉), XIAOJian-zhong(肖建中), WANGCheng-hui(王成辉), HUJin-qiang(胡金强), LAOShen-ying(劳沈颖), YELi-ping(叶丽平), XIANGSong-ping(项松平), LIUQing-e(刘青娥)
Format: Article
Language:zho
Published: Zhejiang University Press 2009-05-01
Series:Zhejiang Daxue xuebao. Lixue ban
Subjects:
Online Access:https://doi.org/10.3785/j.issn.1008-9497.2009.03.018