High-Sensitivity ITS Real-Time PCR Assays for Detection of Ceratocystis lukuohia and Ceratocystis huliohia in Soil and Air Samples

Rapid Ohia Death (ROD) is caused by two pathogens, Ceratocystis lukuohia and Ceratocystis huliohia. The established species-specific real-time PCR assays targeting the single-copy cerato-platanin gene require DNA from between 2 and 16 spores per reaction for consistent detection, which is suitable f...

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Bibliographic Details
Main Authors: Wade P. Heller, Thomas C. Harrington, Eva Brill, Lisa M. Keith
Format: Article
Language:English
Published: The American Phytopathological Society 2023-06-01
Series:PhytoFrontiers
Subjects:
Online Access:https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-09-22-0091-FI