High-Sensitivity ITS Real-Time PCR Assays for Detection of Ceratocystis lukuohia and Ceratocystis huliohia in Soil and Air Samples
Rapid Ohia Death (ROD) is caused by two pathogens, Ceratocystis lukuohia and Ceratocystis huliohia. The established species-specific real-time PCR assays targeting the single-copy cerato-platanin gene require DNA from between 2 and 16 spores per reaction for consistent detection, which is suitable f...
Main Authors: | Wade P. Heller, Thomas C. Harrington, Eva Brill, Lisa M. Keith |
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Format: | Article |
Language: | English |
Published: |
The American Phytopathological Society
2023-06-01
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Series: | PhytoFrontiers |
Subjects: | |
Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-09-22-0091-FI |
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