Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells

Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells

Summary: Vγ9Vδ2 T cells are non-canonical T cells that use their T cell receptor to detect phosphoantigens bound to the internal domain of the HMBPP receptor (butyrophilin 3/2A1 complex). This protocol describes the expansion and purification of human effector Vγ9Vδ2 T cells from human buffy coat an...

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Bibliographic Details
Main Authors: Chia-Hung Christine Hsiao, Andrew J. Wiemer
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Cell-based Assays
Cell separation/fractionation
Cancer
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722003021

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http://www.sciencedirect.com/science/article/pii/S2666166722003021

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