Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells
Summary: Vγ9Vδ2 T cells are non-canonical T cells that use their T cell receptor to detect phosphoantigens bound to the internal domain of the HMBPP receptor (butyrophilin 3/2A1 complex). This protocol describes the expansion and purification of human effector Vγ9Vδ2 T cells from human buffy coat an...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722003021 |
| _version_ | 1818466519411064832 |
|---|---|
| author | Chia-Hung Christine Hsiao Andrew J. Wiemer |
| author_facet | Chia-Hung Christine Hsiao Andrew J. Wiemer |
| author_sort | Chia-Hung Christine Hsiao |
| collection | DOAJ |
| description | Summary: Vγ9Vδ2 T cells are non-canonical T cells that use their T cell receptor to detect phosphoantigens bound to the internal domain of the HMBPP receptor (butyrophilin 3/2A1 complex). This protocol describes the expansion and purification of human effector Vγ9Vδ2 T cells from human buffy coat and describes how to assess their activation by antigen-containing target cells. While specifically focused on cytokine production, this protocol can be readily adapted to evaluate other effector functions of activated Vγ9Vδ2 T cells.For complete details on the use and execution of this protocol, please refer to Hsiao et al. (2022) and Hsiao and Wiemer (2018). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T20:59:23Z |
| format | Article |
| id | doaj.art-d78003924caf4ec2b1b8313d5ee12003 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T20:59:23Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-d78003924caf4ec2b1b8313d5ee120032022-12-22T02:30:12ZengElsevierSTAR Protocols2666-16672022-06-0132101422Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cellsChia-Hung Christine Hsiao0Andrew J. Wiemer1Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269, USA; Corresponding authorDepartment of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269, USA; Institute for Systems Genomics, University of Connecticut, Storrs, CT 06269, USA; Corresponding authorSummary: Vγ9Vδ2 T cells are non-canonical T cells that use their T cell receptor to detect phosphoantigens bound to the internal domain of the HMBPP receptor (butyrophilin 3/2A1 complex). This protocol describes the expansion and purification of human effector Vγ9Vδ2 T cells from human buffy coat and describes how to assess their activation by antigen-containing target cells. While specifically focused on cytokine production, this protocol can be readily adapted to evaluate other effector functions of activated Vγ9Vδ2 T cells.For complete details on the use and execution of this protocol, please refer to Hsiao et al. (2022) and Hsiao and Wiemer (2018). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722003021Cell BiologyCell cultureCell isolationCell-based AssaysCell separation/fractionationCancer |
| spellingShingle | Chia-Hung Christine Hsiao Andrew J. Wiemer Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells STAR Protocols Cell Biology Cell culture Cell isolation Cell-based Assays Cell separation/fractionation Cancer |
| title | Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells |
| title_full | Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells |
| title_fullStr | Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells |
| title_full_unstemmed | Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells |
| title_short | Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells |
| title_sort | generation of effector vγ9vδ2 t cells and evaluation of their response to phosphoantigen loaded cells |
| topic | Cell Biology Cell culture Cell isolation Cell-based Assays Cell separation/fractionation Cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722003021 |
| work_keys_str_mv | AT chiahungchristinehsiao generationofeffectorvg9vd2tcellsandevaluationoftheirresponsetophosphoantigenloadedcells AT andrewjwiemer generationofeffectorvg9vd2tcellsandevaluationoftheirresponsetophosphoantigenloadedcells |