A protocol for identifying the binding sites of small molecules on the cystic fibrosis transmembrane conductance regulator (CFTR) protein

A protocol for identifying the binding sites of small molecules on the cystic fibrosis transmembrane conductance regulator (CFTR) protein

Summary: We describe a protocol to identify the binding site(s) for a drug called ivacaftor that potentiates the CFTR chloride channel. We use photoaffinity probes—based on the structure of ivacaftor—to covalently modify the CFTR protein at the region that constitutes the drug binding site(s). We de...

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Bibliographic Details
Main Authors: Onofrio Laselva, Evgeniy V. Petrotchenko, C. Michael Hamilton, Zafar Qureshi, Christoph H. Borchers, Robert N. Young, Christine E. Bear
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell Membrane
Cell-based Assays
Molecular/Chemical Probes
Protein Biochemistry
Mass Spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001381

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http://www.sciencedirect.com/science/article/pii/S2666166722001381

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