Validation and implementation of a direct RT-qPCR method for rapid screening of SARS-CoV-2 infection by using non-invasive saliva samples

Validation and implementation of a direct RT-qPCR method for rapid screening of SARS-CoV-2 infection by using non-invasive saliva samples

Objective: To validate and implement an optimized screening method for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA combining use of self-collected raw saliva samples, single-step heat-treated virus inactivation and RNA extraction, and direct RT-qPCR. Methods: Th...

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Bibliographic Details
Main Authors: Pedro Brotons, Amaresh Perez-Argüello, Cristian Launes, Francesc Torrents, Maria Pilar Subirats, Jesica Saucedo, Joana Claverol, Juan Jose Garcia-Garcia, Gil Rodas, Vicky Fumado, Iolanda Jordan, Eduard Gratacos, Quique Bassat, Carmen Muñoz-Almagro
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:International Journal of Infectious Diseases
Subjects:
SARS-CoV-2
COVID-19
PCR
Saliva
Screening
Surveillance
Online Access:http://www.sciencedirect.com/science/article/pii/S120197122100607X

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http://www.sciencedirect.com/science/article/pii/S120197122100607X

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