CRISPR-Cas9 Toolkit for Genome Editing in an Autotrophic CO2-Fixing Methanogenic Archaeon

CRISPR-Cas9 Toolkit for Genome Editing in an Autotrophic CO2-Fixing Methanogenic Archaeon

ABSTRACT The CRISPR-Cas9 system is a robust genome editing tool that is widely applied in eukaryotes and bacteria. However, use of this technique has only been developed for one species of Archaea, a domain of life ranking in parallel with Eukarya and Bacteria. In this study, we applied the CRISPR-C...

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Bibliographic Details
Main Authors: Jie Li, Liuyang Zhang, Qing Xu, Wenting Zhang, Zhihua Li, Lei Chen, Xiuzhu Dong
Format: Article
Language:English
Published: American Society for Microbiology 2022-08-01
Series:Microbiology Spectrum
Subjects:
CRISPR-Cas9
genome editing
archaea
CO2 fixing
methanogenic archaeon
genetics
Online Access:https://journals.asm.org/doi/10.1128/spectrum.01165-22

Internet

https://journals.asm.org/doi/10.1128/spectrum.01165-22

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