Direct Infusion Mass Spectrometry to Rapidly Map Metabolic Flux of Substrates Labeled with Stable Isotopes

Direct Infusion Mass Spectrometry to Rapidly Map Metabolic Flux of Substrates Labeled with Stable Isotopes

Direct infusion–high-resolution mass spectrometry (DI-HRMS) allows for rapid profiling of complex mixtures of metabolites in blood, cerebrospinal fluid, tissue samples and cultured cells. Here, we present a DI-HRMS method suitable for the rapid determination of metabolic fluxes of isotopically label...

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Bibliographic Details
Main Authors: Nils W. F. Meijer, Susan Zwakenberg, Johan Gerrits, Denise Westland, Arif I. Ardisasmita, Sabine A. Fuchs, Nanda M. Verhoeven-Duif, Judith J. M. Jans, Fried J. T. Zwartkruis
Format: Article
Language:English
Published: MDPI AG 2024-04-01
Series:Metabolites
Subjects:
direct infusion–high-resolution mass spectrometry
isotope tracing
glycolysis
TCA cycle
glutaminolysis
organoids
Online Access:https://www.mdpi.com/2218-1989/14/5/246

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https://www.mdpi.com/2218-1989/14/5/246

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