Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis
Immunosenescence and inflammaging facilitate the insurgence of chronic diseases. The Mediterranean diet is a non-invasive intervention to improve the chronic low-grade inflammatory status associated with aging. Olive oil oleuropein (OLE) and hydroxytyrosol (HT) demonstrated a controversial modulator...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/13/11029 |
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| author | Fanny Pojero Francesco Gervasi Salvatore Davide Fiore Anna Aiello Sonia Bonacci Rosalia Caldarella Alessandro Attanzio Giuseppina Candore Calogero Caruso Mattia Emanuela Ligotti Antonio Procopio Ignazio Restivo Luisa Tesoriere Mario Allegra Giulia Accardi |
| author_facet | Fanny Pojero Francesco Gervasi Salvatore Davide Fiore Anna Aiello Sonia Bonacci Rosalia Caldarella Alessandro Attanzio Giuseppina Candore Calogero Caruso Mattia Emanuela Ligotti Antonio Procopio Ignazio Restivo Luisa Tesoriere Mario Allegra Giulia Accardi |
| author_sort | Fanny Pojero |
| collection | DOAJ |
| description | Immunosenescence and inflammaging facilitate the insurgence of chronic diseases. The Mediterranean diet is a non-invasive intervention to improve the chronic low-grade inflammatory status associated with aging. Olive oil oleuropein (OLE) and hydroxytyrosol (HT) demonstrated a controversial modulatory action on inflammation in vitro when tested at concentrations exceeding those detectable in human plasma. We studied the potential anti-inflammatory effects of OLE and HT at nutritionally relevant concentrations on peripheral blood mononuclear cells (PBMCs) as regards cell viability, frequency of leukocyte subsets, and cytokine release, performing an age-focused analysis on two groups of subjects: Adult (age 18–64 years) and Senior (age ≥ 65 years). OLE and HT were used alone or as a pre-treatment before challenging PBMCs with lipopolysaccharide (LPS). Both polyphenols had no effect on cell viability irrespective of LPS, but 5 µM HT had an LPS-like effect on monocytes, reducing the intermediate subset in Adult subjects. OLE and HT had no effect on LPS-triggered release of TNF-α, IL-6 and IL-8, but 5 µM HT reduced IL-10 secretion by PBMCs from Adult vs. Senior group. In summary, nutritionally relevant concentrations of OLE and HT elicit no anti-inflammatory effect and influence the frequency of immune cell subsets with age-related different outcomes. |
| first_indexed | 2024-03-11T01:38:31Z |
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| id | doaj.art-d7b5643fe7e74ac080eb9c6bde7054fc |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:38:31Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d7b5643fe7e74ac080eb9c6bde7054fc2023-11-18T16:47:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124131102910.3390/ijms241311029Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related AnalysisFanny Pojero0Francesco Gervasi1Salvatore Davide Fiore2Anna Aiello3Sonia Bonacci4Rosalia Caldarella5Alessandro Attanzio6Giuseppina Candore7Calogero Caruso8Mattia Emanuela Ligotti9Antonio Procopio10Ignazio Restivo11Luisa Tesoriere12Mario Allegra13Giulia Accardi14Laboratory of Immunopathology and Immunosenescence, Department of Biomedicine, Neurosciences and Advanced Technologies, University of Palermo, 90133 Palermo, ItalySpecialistic Oncology Laboratory Unit, ARNAS Hospitals Civico Di Cristina e Benfratelli, 90127 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, 90123 Palermo, ItalyLaboratory of Immunopathology and Immunosenescence, Department of Biomedicine, Neurosciences and Advanced Technologies, University of Palermo, 90133 Palermo, ItalyDepartment of Health Sciences, University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Laboratory Medicine, “P. Giaccone” University Hospital, 90127 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, 90123 Palermo, ItalyLaboratory of Immunopathology and Immunosenescence, Department of Biomedicine, Neurosciences and Advanced Technologies, University of Palermo, 90133 Palermo, ItalyLaboratory of Immunopathology and Immunosenescence, Department of Biomedicine, Neurosciences and Advanced Technologies, University of Palermo, 90133 Palermo, ItalyLaboratory of Immunopathology and Immunosenescence, Department of Biomedicine, Neurosciences and Advanced Technologies, University of Palermo, 90133 Palermo, ItalyDepartment of Health Sciences, University Magna Graecia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, 90123 Palermo, ItalyLaboratory of Immunopathology and Immunosenescence, Department of Biomedicine, Neurosciences and Advanced Technologies, University of Palermo, 90133 Palermo, ItalyImmunosenescence and inflammaging facilitate the insurgence of chronic diseases. The Mediterranean diet is a non-invasive intervention to improve the chronic low-grade inflammatory status associated with aging. Olive oil oleuropein (OLE) and hydroxytyrosol (HT) demonstrated a controversial modulatory action on inflammation in vitro when tested at concentrations exceeding those detectable in human plasma. We studied the potential anti-inflammatory effects of OLE and HT at nutritionally relevant concentrations on peripheral blood mononuclear cells (PBMCs) as regards cell viability, frequency of leukocyte subsets, and cytokine release, performing an age-focused analysis on two groups of subjects: Adult (age 18–64 years) and Senior (age ≥ 65 years). OLE and HT were used alone or as a pre-treatment before challenging PBMCs with lipopolysaccharide (LPS). Both polyphenols had no effect on cell viability irrespective of LPS, but 5 µM HT had an LPS-like effect on monocytes, reducing the intermediate subset in Adult subjects. OLE and HT had no effect on LPS-triggered release of TNF-α, IL-6 and IL-8, but 5 µM HT reduced IL-10 secretion by PBMCs from Adult vs. Senior group. In summary, nutritionally relevant concentrations of OLE and HT elicit no anti-inflammatory effect and influence the frequency of immune cell subsets with age-related different outcomes.https://www.mdpi.com/1422-0067/24/13/11029oleuropeinhydroxytyrosolPBMCLPSinflammaging |
| spellingShingle | Fanny Pojero Francesco Gervasi Salvatore Davide Fiore Anna Aiello Sonia Bonacci Rosalia Caldarella Alessandro Attanzio Giuseppina Candore Calogero Caruso Mattia Emanuela Ligotti Antonio Procopio Ignazio Restivo Luisa Tesoriere Mario Allegra Giulia Accardi Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis International Journal of Molecular Sciences oleuropein hydroxytyrosol PBMC LPS inflammaging |
| title | Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis |
| title_full | Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis |
| title_fullStr | Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis |
| title_full_unstemmed | Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis |
| title_short | Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis |
| title_sort | anti inflammatory effects of nutritionally relevant concentrations of oleuropein and hydroxytyrosol on peripheral blood mononuclear cells an age related analysis |
| topic | oleuropein hydroxytyrosol PBMC LPS inflammaging |
| url | https://www.mdpi.com/1422-0067/24/13/11029 |
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