Mapping of Genetic Locus for Leaf Trichome Formation in Chinese Cabbage Based on Bulked Segregant Analysis
Chinese cabbage is a leafy vegetable, and its leaves are the main edible organs. The formation of trichomes on the leaves can significantly affect its taste, so studying this phenomenon is of great significance for improving the quality of Chinese cabbage. In this study, two varieties of Chinese cab...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/10/4/771 |
| _version_ | 1797537748963295232 |
|---|---|
| author | Rujia Zhang Yiming Ren Huiyuan Wu Yu Yang Mengguo Yuan Haonan Liang Changwei Zhang |
| author_facet | Rujia Zhang Yiming Ren Huiyuan Wu Yu Yang Mengguo Yuan Haonan Liang Changwei Zhang |
| author_sort | Rujia Zhang |
| collection | DOAJ |
| description | Chinese cabbage is a leafy vegetable, and its leaves are the main edible organs. The formation of trichomes on the leaves can significantly affect its taste, so studying this phenomenon is of great significance for improving the quality of Chinese cabbage. In this study, two varieties of Chinese cabbage, W30 with trichome leaves and 082 with glabrous leaves, were crossed to generate F<sub>1</sub> and F<sub>1</sub> plants, which were self-fertilized to develop segregating populations with trichome or glabrous morphotypes. The two bulks of the different segregating populations were used to conduct bulked segregant analysis (BSA). A total of 293.4 M clean reads were generated from the samples, and plants from the trichome leaves (AL) bulk and glabrous leaves (GL) bulk were identified. Between the two DNA pools generated from the trichome and glabrous plants, 55,048 SNPs and 272 indels were generated. In this study, three regions (on chromosomes 6, 10 and scaffold000100) were identified, and the annotation revealed three candidate genes that may participate in the formation of leaf trichomes. These findings suggest that the three genes—<i>Bra025087</i> encoding a cyclin family protein, <i>Bra035000</i> encoding an ATP-binding protein/kinase/protein kinase/protein serine/threonine kinase and <i>Bra033370</i> encoding a WD-40 repeat family protein–influence the formation of trichomes by participating in trichome morphogenesis (GO: 0010090). These results demonstrate that BSA can be used to map genes associated with traits and provide new insights into the molecular mechanism of leafy trichome formation in Chinese cabbage. |
| first_indexed | 2024-03-10T12:19:46Z |
| format | Article |
| id | doaj.art-d7c56a2f435948ccb316941f69d568e4 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T12:19:46Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-d7c56a2f435948ccb316941f69d568e42023-11-21T15:34:59ZengMDPI AGPlants2223-77472021-04-0110477110.3390/plants10040771Mapping of Genetic Locus for Leaf Trichome Formation in Chinese Cabbage Based on Bulked Segregant AnalysisRujia Zhang0Yiming Ren1Huiyuan Wu2Yu Yang3Mengguo Yuan4Haonan Liang5Changwei Zhang6State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaChinese cabbage is a leafy vegetable, and its leaves are the main edible organs. The formation of trichomes on the leaves can significantly affect its taste, so studying this phenomenon is of great significance for improving the quality of Chinese cabbage. In this study, two varieties of Chinese cabbage, W30 with trichome leaves and 082 with glabrous leaves, were crossed to generate F<sub>1</sub> and F<sub>1</sub> plants, which were self-fertilized to develop segregating populations with trichome or glabrous morphotypes. The two bulks of the different segregating populations were used to conduct bulked segregant analysis (BSA). A total of 293.4 M clean reads were generated from the samples, and plants from the trichome leaves (AL) bulk and glabrous leaves (GL) bulk were identified. Between the two DNA pools generated from the trichome and glabrous plants, 55,048 SNPs and 272 indels were generated. In this study, three regions (on chromosomes 6, 10 and scaffold000100) were identified, and the annotation revealed three candidate genes that may participate in the formation of leaf trichomes. These findings suggest that the three genes—<i>Bra025087</i> encoding a cyclin family protein, <i>Bra035000</i> encoding an ATP-binding protein/kinase/protein kinase/protein serine/threonine kinase and <i>Bra033370</i> encoding a WD-40 repeat family protein–influence the formation of trichomes by participating in trichome morphogenesis (GO: 0010090). These results demonstrate that BSA can be used to map genes associated with traits and provide new insights into the molecular mechanism of leafy trichome formation in Chinese cabbage.https://www.mdpi.com/2223-7747/10/4/771bulked segregant analysisChinese cabbageleaf trichome |
| spellingShingle | Rujia Zhang Yiming Ren Huiyuan Wu Yu Yang Mengguo Yuan Haonan Liang Changwei Zhang Mapping of Genetic Locus for Leaf Trichome Formation in Chinese Cabbage Based on Bulked Segregant Analysis Plants bulked segregant analysis Chinese cabbage leaf trichome |
| title | Mapping of Genetic Locus for Leaf Trichome Formation in Chinese Cabbage Based on Bulked Segregant Analysis |
| title_full | Mapping of Genetic Locus for Leaf Trichome Formation in Chinese Cabbage Based on Bulked Segregant Analysis |
| title_fullStr | Mapping of Genetic Locus for Leaf Trichome Formation in Chinese Cabbage Based on Bulked Segregant Analysis |
| title_full_unstemmed | Mapping of Genetic Locus for Leaf Trichome Formation in Chinese Cabbage Based on Bulked Segregant Analysis |
| title_short | Mapping of Genetic Locus for Leaf Trichome Formation in Chinese Cabbage Based on Bulked Segregant Analysis |
| title_sort | mapping of genetic locus for leaf trichome formation in chinese cabbage based on bulked segregant analysis |
| topic | bulked segregant analysis Chinese cabbage leaf trichome |
| url | https://www.mdpi.com/2223-7747/10/4/771 |
| work_keys_str_mv | AT rujiazhang mappingofgeneticlocusforleaftrichomeformationinchinesecabbagebasedonbulkedsegregantanalysis AT yimingren mappingofgeneticlocusforleaftrichomeformationinchinesecabbagebasedonbulkedsegregantanalysis AT huiyuanwu mappingofgeneticlocusforleaftrichomeformationinchinesecabbagebasedonbulkedsegregantanalysis AT yuyang mappingofgeneticlocusforleaftrichomeformationinchinesecabbagebasedonbulkedsegregantanalysis AT mengguoyuan mappingofgeneticlocusforleaftrichomeformationinchinesecabbagebasedonbulkedsegregantanalysis AT haonanliang mappingofgeneticlocusforleaftrichomeformationinchinesecabbagebasedonbulkedsegregantanalysis AT changweizhang mappingofgeneticlocusforleaftrichomeformationinchinesecabbagebasedonbulkedsegregantanalysis |