Protocol for purifying biologically active microtubule-severing protein UNC-45A from E.coli using GST-affinity and spin columns

Summary: Recombinant microtubule (MT)-severing proteins are valuable for studying their mechanisms of action; however, purifying them in an active state is challenging. Here, we provide a protocol to obtain biologically active and highly pure recombinant GFP-UNC-45A, a novel ATP-independent MT-sever...

Täydet tiedot

Bibliografiset tiedot
Päätekijät: Asumi Hoshino, Nimisha Krishnan, Mihir Shetty, Jennifer L. Ross, Martina Bazzaro
Aineistotyyppi: Artikkeli
Kieli:English
Julkaistu: Elsevier 2025-03-01
Sarja:STAR Protocols
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Linkit:http://www.sciencedirect.com/science/article/pii/S2666166725000619