All-optical interrogation of brain-wide activity in freely swimming larval zebrafish

Summary: We introduce an all-optical technique that enables volumetric imaging of brain-wide calcium activity and targeted optogenetic stimulation of specific brain regions in unrestrained larval zebrafish. The system consists of three main components: a 3D tracking module, a dual-color fluorescence...

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Bibliographic Details
Main Authors: Yuming Chai, Kexin Qi, Yubin Wu, Daguang Li, Guodong Tan, Yuqi Guo, Jun Chu, Yu Mu, Chen Shen, Quan Wen
Format: Article
Language:English
Published: Elsevier 2024-01-01
Series:iScience
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004223024628