All-optical interrogation of brain-wide activity in freely swimming larval zebrafish
Summary: We introduce an all-optical technique that enables volumetric imaging of brain-wide calcium activity and targeted optogenetic stimulation of specific brain regions in unrestrained larval zebrafish. The system consists of three main components: a 3D tracking module, a dual-color fluorescence...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223024628 |
| _version_ | 1797378633866674176 |
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| author | Yuming Chai Kexin Qi Yubin Wu Daguang Li Guodong Tan Yuqi Guo Jun Chu Yu Mu Chen Shen Quan Wen |
| author_facet | Yuming Chai Kexin Qi Yubin Wu Daguang Li Guodong Tan Yuqi Guo Jun Chu Yu Mu Chen Shen Quan Wen |
| author_sort | Yuming Chai |
| collection | DOAJ |
| description | Summary: We introduce an all-optical technique that enables volumetric imaging of brain-wide calcium activity and targeted optogenetic stimulation of specific brain regions in unrestrained larval zebrafish. The system consists of three main components: a 3D tracking module, a dual-color fluorescence imaging module, and a real-time activity manipulation module. Our approach uses a sensitive genetically encoded calcium indicator in combination with a long Stokes shift red fluorescence protein as a reference channel, allowing the extraction of Ca2+ activity from signals contaminated by motion artifacts. The method also incorporates rapid 3D image reconstruction and registration, facilitating real-time selective optogenetic stimulation of different regions of the brain. By demonstrating that selective light activation of the midbrain regions in larval zebrafish could reliably trigger biased turning behavior and changes of brain-wide neural activity, we present a valuable tool for investigating the causal relationship between distributed neural circuit dynamics and naturalistic behavior. |
| first_indexed | 2024-03-08T20:10:35Z |
| format | Article |
| id | doaj.art-d7e31c2034f9477fbc1b7d343b9d603e |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T20:10:35Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-d7e31c2034f9477fbc1b7d343b9d603e2023-12-23T05:22:25ZengElsevieriScience2589-00422024-01-01271108385All-optical interrogation of brain-wide activity in freely swimming larval zebrafishYuming Chai0Kexin Qi1Yubin Wu2Daguang Li3Guodong Tan4Yuqi Guo5Jun Chu6Yu Mu7Chen Shen8Quan Wen9Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China; Hefei National Research Center for Physical Sciences at the Microscale, Center for Integrative Imaging, University of Science and Technology of China, Hefei, China; Corresponding authorDivision of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China; Hefei National Research Center for Physical Sciences at the Microscale, Center for Integrative Imaging, University of Science and Technology of China, Hefei, ChinaDivision of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China; Hefei National Research Center for Physical Sciences at the Microscale, Center for Integrative Imaging, University of Science and Technology of China, Hefei, ChinaDivision of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China; Hefei National Research Center for Physical Sciences at the Microscale, Center for Integrative Imaging, University of Science and Technology of China, Hefei, ChinaDivision of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China; Hefei National Research Center for Physical Sciences at the Microscale, Center for Integrative Imaging, University of Science and Technology of China, Hefei, ChinaGuangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology and Center for Biomedical Optics and Molecular Imaging, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, ChinaGuangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology and Center for Biomedical Optics and Molecular Imaging, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, ChinaInstitute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, ChinaDivision of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China; Hefei National Research Center for Physical Sciences at the Microscale, Center for Integrative Imaging, University of Science and Technology of China, Hefei, China; Corresponding authorDivision of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China; Hefei National Research Center for Physical Sciences at the Microscale, Center for Integrative Imaging, University of Science and Technology of China, Hefei, China; Corresponding authorSummary: We introduce an all-optical technique that enables volumetric imaging of brain-wide calcium activity and targeted optogenetic stimulation of specific brain regions in unrestrained larval zebrafish. The system consists of three main components: a 3D tracking module, a dual-color fluorescence imaging module, and a real-time activity manipulation module. Our approach uses a sensitive genetically encoded calcium indicator in combination with a long Stokes shift red fluorescence protein as a reference channel, allowing the extraction of Ca2+ activity from signals contaminated by motion artifacts. The method also incorporates rapid 3D image reconstruction and registration, facilitating real-time selective optogenetic stimulation of different regions of the brain. By demonstrating that selective light activation of the midbrain regions in larval zebrafish could reliably trigger biased turning behavior and changes of brain-wide neural activity, we present a valuable tool for investigating the causal relationship between distributed neural circuit dynamics and naturalistic behavior.http://www.sciencedirect.com/science/article/pii/S2589004223024628NeuroscienceBehavioral neuroscienceBiocomputational method |
| spellingShingle | Yuming Chai Kexin Qi Yubin Wu Daguang Li Guodong Tan Yuqi Guo Jun Chu Yu Mu Chen Shen Quan Wen All-optical interrogation of brain-wide activity in freely swimming larval zebrafish iScience Neuroscience Behavioral neuroscience Biocomputational method |
| title | All-optical interrogation of brain-wide activity in freely swimming larval zebrafish |
| title_full | All-optical interrogation of brain-wide activity in freely swimming larval zebrafish |
| title_fullStr | All-optical interrogation of brain-wide activity in freely swimming larval zebrafish |
| title_full_unstemmed | All-optical interrogation of brain-wide activity in freely swimming larval zebrafish |
| title_short | All-optical interrogation of brain-wide activity in freely swimming larval zebrafish |
| title_sort | all optical interrogation of brain wide activity in freely swimming larval zebrafish |
| topic | Neuroscience Behavioral neuroscience Biocomputational method |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223024628 |
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