D-galactan II is an immunodominant antigen in O1 lipopolysaccharide and affects virulence in Klebsiella pneumoniae: implication in vaccine design
In the O1 strain of Klebsiella, the lipopolysaccharide (LPS) O-antigen is composed of D-galactan I and D-galactan II. Although the composition of the O1 antigen of Klebsiella was resolved more than two decades, the genetic locus involved in the biosynthesis of D-galactan II and the role of D-galacta...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00608/full |
| _version_ | 1818296082377998336 |
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| author | Pei-Fang eHsieh Meng-Chuan eWu Feng-Ling eYang Chun-Tang eChen Tzu-Chi eLou Yi-Yin eChen Shih-Hsiung eWu Jin-Chuan eSheu Jin-Town eWang Jin-Town eWang |
| author_facet | Pei-Fang eHsieh Meng-Chuan eWu Feng-Ling eYang Chun-Tang eChen Tzu-Chi eLou Yi-Yin eChen Shih-Hsiung eWu Jin-Chuan eSheu Jin-Town eWang Jin-Town eWang |
| author_sort | Pei-Fang eHsieh |
| collection | DOAJ |
| description | In the O1 strain of Klebsiella, the lipopolysaccharide (LPS) O-antigen is composed of D-galactan I and D-galactan II. Although the composition of the O1 antigen of Klebsiella was resolved more than two decades, the genetic locus involved in the biosynthesis of D-galactan II and the role of D-galactan II in bacterial pathogenesis remain unclear. Here, we report the identification of the D-galactan II-synthesizing genes by screening a transposon mutant library of an acapsulated Klebsiella pneumoniae O1 strain with bacteriophage. K. pneumoniae strain deleted for wbbY exhibited abrogated D-galactan II production; altered serum resistance and attenuation of virulence. Serologic analysis of K. pneumoniae clinical isolates demonstrated that D-galactan II was more prevalent in community-acquired pyogenic liver abscess (PLA) -causing strains than in non-tissue-invasive strains. WbbY homologs, WbbZ homologs, and lipopolysaccharide structures based on D-galactan II also were present in several Gram-negative bacteria. Immunization of mice with the magA-mutant (K1- O1) (that is, with a LPS D-galactan II-producing strain) provided protection against infection with an O1:K2 PLA strain. Our findings indicate that both WbbY and WbbZ homologs are sufficient for the synthesis of D-galactan II. D-galactan II represents an immunodominant antigen; is conserved among multiple species of Gram-negative bacteria and could be a useful vaccine candidate. |
| first_indexed | 2024-12-13T03:57:53Z |
| format | Article |
| id | doaj.art-d7e42e525e704f888051e40aff224d2d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T03:57:53Z |
| publishDate | 2014-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d7e42e525e704f888051e40aff224d2d2022-12-22T00:00:35ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2014-11-01510.3389/fmicb.2014.00608119214D-galactan II is an immunodominant antigen in O1 lipopolysaccharide and affects virulence in Klebsiella pneumoniae: implication in vaccine designPei-Fang eHsieh0Meng-Chuan eWu1Feng-Ling eYang2Chun-Tang eChen3Tzu-Chi eLou4Yi-Yin eChen5Shih-Hsiung eWu6Jin-Chuan eSheu7Jin-Town eWang8Jin-Town eWang9Dept. of Microbiology, National Taiwan University College of MedicineDepartment of Internal Medicine, National Taiwan University HospitalThe Institute of Biological Chemistry, Academia SinicaDept. of Microbiology, National Taiwan University College of MedicineThe Institute of Biological Chemistry, Academia SinicaDept. of Microbiology, National Taiwan University College of MedicineThe Institute of Biological Chemistry, Academia SinicaDepartment of Internal Medicine, National Taiwan University HospitalDept. of Microbiology, National Taiwan University College of MedicineDepartment of Internal Medicine, National Taiwan University HospitalIn the O1 strain of Klebsiella, the lipopolysaccharide (LPS) O-antigen is composed of D-galactan I and D-galactan II. Although the composition of the O1 antigen of Klebsiella was resolved more than two decades, the genetic locus involved in the biosynthesis of D-galactan II and the role of D-galactan II in bacterial pathogenesis remain unclear. Here, we report the identification of the D-galactan II-synthesizing genes by screening a transposon mutant library of an acapsulated Klebsiella pneumoniae O1 strain with bacteriophage. K. pneumoniae strain deleted for wbbY exhibited abrogated D-galactan II production; altered serum resistance and attenuation of virulence. Serologic analysis of K. pneumoniae clinical isolates demonstrated that D-galactan II was more prevalent in community-acquired pyogenic liver abscess (PLA) -causing strains than in non-tissue-invasive strains. WbbY homologs, WbbZ homologs, and lipopolysaccharide structures based on D-galactan II also were present in several Gram-negative bacteria. Immunization of mice with the magA-mutant (K1- O1) (that is, with a LPS D-galactan II-producing strain) provided protection against infection with an O1:K2 PLA strain. Our findings indicate that both WbbY and WbbZ homologs are sufficient for the synthesis of D-galactan II. D-galactan II represents an immunodominant antigen; is conserved among multiple species of Gram-negative bacteria and could be a useful vaccine candidate.http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00608/fullKlebsiella pneumoniaelipopolysaccharideVaccineD-galactan IIimmunodominant antigen |
| spellingShingle | Pei-Fang eHsieh Meng-Chuan eWu Feng-Ling eYang Chun-Tang eChen Tzu-Chi eLou Yi-Yin eChen Shih-Hsiung eWu Jin-Chuan eSheu Jin-Town eWang Jin-Town eWang D-galactan II is an immunodominant antigen in O1 lipopolysaccharide and affects virulence in Klebsiella pneumoniae: implication in vaccine design Frontiers in Microbiology Klebsiella pneumoniae lipopolysaccharide Vaccine D-galactan II immunodominant antigen |
| title | D-galactan II is an immunodominant antigen in O1 lipopolysaccharide and affects virulence in Klebsiella pneumoniae: implication in vaccine design |
| title_full | D-galactan II is an immunodominant antigen in O1 lipopolysaccharide and affects virulence in Klebsiella pneumoniae: implication in vaccine design |
| title_fullStr | D-galactan II is an immunodominant antigen in O1 lipopolysaccharide and affects virulence in Klebsiella pneumoniae: implication in vaccine design |
| title_full_unstemmed | D-galactan II is an immunodominant antigen in O1 lipopolysaccharide and affects virulence in Klebsiella pneumoniae: implication in vaccine design |
| title_short | D-galactan II is an immunodominant antigen in O1 lipopolysaccharide and affects virulence in Klebsiella pneumoniae: implication in vaccine design |
| title_sort | d galactan ii is an immunodominant antigen in o1 lipopolysaccharide and affects virulence in klebsiella pneumoniae implication in vaccine design |
| topic | Klebsiella pneumoniae lipopolysaccharide Vaccine D-galactan II immunodominant antigen |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00608/full |
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