Preharvest UVB Application Increases Glucosinolate Contents and Enhances Postharvest Quality of Broccoli Microgreens
Broccoli microgreens have shown potential health benefits due to their high glucosinolate (GL) levels. Previously, we observed that postharvest UVB treatment did not have much effect on increasing GLs in broccoli microgreens. In this study, we investigated the influence of preharvest UVB irradiation...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/11/3247 |
| _version_ | 1827691235803922432 |
|---|---|
| author | Yingjian Lu Wen Dong Tianbao Yang Yaguang Luo Pei Chen |
| author_facet | Yingjian Lu Wen Dong Tianbao Yang Yaguang Luo Pei Chen |
| author_sort | Yingjian Lu |
| collection | DOAJ |
| description | Broccoli microgreens have shown potential health benefits due to their high glucosinolate (GL) levels. Previously, we observed that postharvest UVB treatment did not have much effect on increasing GLs in broccoli microgreens. In this study, we investigated the influence of preharvest UVB irradiation on GL levels in broccoli microgreens. UHPLC-ESI/ITMS analysis showed that preharvest UVB treatments with UVB 0.09 and 0.27 Wh/m<sup>2</sup> significantly increased the glucoraphanin (GLR), glucoerucin (GLE), and total aliphatic GL levels by 13.7 and 16.9%, respectively, in broccoli microgreens when measured on harvest day. The nutritional qualities of UVB-treated microgreens were stable during 21-day storage, with only small changes in their GL levels. Broccoli microgreens treated before harvest with UVB 0.27 Wh/m<sup>2</sup> and 10 mM CaCl<sub>2</sub> spray maintained their overall quality, and had the lowest tissue electrolyte leakage and off-odor values during the storage. Furthermore, preharvest UVB 0.27 Wh/m<sup>2</sup> treatment significantly increased GL biosynthesis genes when evaluated before harvest, and reduced the expression level of myrosinase, a gene responsible for GL breakdown during postharvest storage. Overall, preharvest UVB treatment, together with calcium chloride spray, can increase and maintain health-beneficial compound levels such as GLs and prolong the postharvest quality of broccoli microgreens. |
| first_indexed | 2024-03-10T10:56:59Z |
| format | Article |
| id | doaj.art-d7ee1cdcaeea40fbbf70f967e17ba056 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:56:59Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d7ee1cdcaeea40fbbf70f967e17ba0562023-11-21T21:50:00ZengMDPI AGMolecules1420-30492021-05-012611324710.3390/molecules26113247Preharvest UVB Application Increases Glucosinolate Contents and Enhances Postharvest Quality of Broccoli MicrogreensYingjian Lu0Wen Dong1Tianbao Yang2Yaguang Luo3Pei Chen4College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210095, ChinaBeltsville Agricultural Research Center, Food Quality Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USABeltsville Agricultural Research Center, Food Quality Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USABeltsville Agricultural Research Center, Food Quality Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USABeltsville Human Nutrition Research Center, Methods and Application of Food Composition Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USABroccoli microgreens have shown potential health benefits due to their high glucosinolate (GL) levels. Previously, we observed that postharvest UVB treatment did not have much effect on increasing GLs in broccoli microgreens. In this study, we investigated the influence of preharvest UVB irradiation on GL levels in broccoli microgreens. UHPLC-ESI/ITMS analysis showed that preharvest UVB treatments with UVB 0.09 and 0.27 Wh/m<sup>2</sup> significantly increased the glucoraphanin (GLR), glucoerucin (GLE), and total aliphatic GL levels by 13.7 and 16.9%, respectively, in broccoli microgreens when measured on harvest day. The nutritional qualities of UVB-treated microgreens were stable during 21-day storage, with only small changes in their GL levels. Broccoli microgreens treated before harvest with UVB 0.27 Wh/m<sup>2</sup> and 10 mM CaCl<sub>2</sub> spray maintained their overall quality, and had the lowest tissue electrolyte leakage and off-odor values during the storage. Furthermore, preharvest UVB 0.27 Wh/m<sup>2</sup> treatment significantly increased GL biosynthesis genes when evaluated before harvest, and reduced the expression level of myrosinase, a gene responsible for GL breakdown during postharvest storage. Overall, preharvest UVB treatment, together with calcium chloride spray, can increase and maintain health-beneficial compound levels such as GLs and prolong the postharvest quality of broccoli microgreens.https://www.mdpi.com/1420-3049/26/11/3247<i>Brassica oleracea</i> var. <i>italica</i>phenolicsfunctional foodcalciumUVB radiation |
| spellingShingle | Yingjian Lu Wen Dong Tianbao Yang Yaguang Luo Pei Chen Preharvest UVB Application Increases Glucosinolate Contents and Enhances Postharvest Quality of Broccoli Microgreens Molecules <i>Brassica oleracea</i> var. <i>italica</i> phenolics functional food calcium UVB radiation |
| title | Preharvest UVB Application Increases Glucosinolate Contents and Enhances Postharvest Quality of Broccoli Microgreens |
| title_full | Preharvest UVB Application Increases Glucosinolate Contents and Enhances Postharvest Quality of Broccoli Microgreens |
| title_fullStr | Preharvest UVB Application Increases Glucosinolate Contents and Enhances Postharvest Quality of Broccoli Microgreens |
| title_full_unstemmed | Preharvest UVB Application Increases Glucosinolate Contents and Enhances Postharvest Quality of Broccoli Microgreens |
| title_short | Preharvest UVB Application Increases Glucosinolate Contents and Enhances Postharvest Quality of Broccoli Microgreens |
| title_sort | preharvest uvb application increases glucosinolate contents and enhances postharvest quality of broccoli microgreens |
| topic | <i>Brassica oleracea</i> var. <i>italica</i> phenolics functional food calcium UVB radiation |
| url | https://www.mdpi.com/1420-3049/26/11/3247 |
| work_keys_str_mv | AT yingjianlu preharvestuvbapplicationincreasesglucosinolatecontentsandenhancespostharvestqualityofbroccolimicrogreens AT wendong preharvestuvbapplicationincreasesglucosinolatecontentsandenhancespostharvestqualityofbroccolimicrogreens AT tianbaoyang preharvestuvbapplicationincreasesglucosinolatecontentsandenhancespostharvestqualityofbroccolimicrogreens AT yaguangluo preharvestuvbapplicationincreasesglucosinolatecontentsandenhancespostharvestqualityofbroccolimicrogreens AT peichen preharvestuvbapplicationincreasesglucosinolatecontentsandenhancespostharvestqualityofbroccolimicrogreens |