Phos-tag-based non-radioactive protocols for monitoring Arabidopsis kinase activities in vitro

Phos-tag-based non-radioactive protocols for monitoring Arabidopsis kinase activities in vitro

Summary: Kinases are indispensable signaling components. Radioactive-based phosphorylation assays are widely used but require specific protective equipment and safety trainings. Here, we present a Phos-tag-based non-radioactive kinase assay to study Arabidopsis kinase activities. We expressed and pu...

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Bibliographic Details
Main Authors: Junjie Shi, Manru Fang, Ran Wang, Ziqiang Zhu
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Model organisms
Plant sciences
Signal transduction
Protein biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722005974

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http://www.sciencedirect.com/science/article/pii/S2666166722005974

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