Phos-tag-based non-radioactive protocols for monitoring Arabidopsis kinase activities in vitro
Summary: Kinases are indispensable signaling components. Radioactive-based phosphorylation assays are widely used but require specific protective equipment and safety trainings. Here, we present a Phos-tag-based non-radioactive kinase assay to study Arabidopsis kinase activities. We expressed and pu...
Main Authors: | Junjie Shi, Manru Fang, Ran Wang, Ziqiang Zhu |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-12-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722005974 |
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