CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

Abstract Background Pichia pastoris (syn. Komagataella phaffii) is a widely used generally recognized as safe host for heterologous expression of proteins in both industry and academia. Recently, it has been shown to be a potentially good chassis host for the production of high-value pharmaceuticals...

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Bibliographic Details
Main Authors: Qi Liu, Xiaona Shi, Lili Song, Haifeng Liu, Xiangshan Zhou, Qiyao Wang, Yuanxing Zhang, Menghao Cai
Format: Article
Language:English
Published: BMC 2019-08-01
Series:Microbial Cell Factories
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12934-019-1194-x