CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris
Abstract Background Pichia pastoris (syn. Komagataella phaffii) is a widely used generally recognized as safe host for heterologous expression of proteins in both industry and academia. Recently, it has been shown to be a potentially good chassis host for the production of high-value pharmaceuticals...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2019-08-01
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Series: | Microbial Cell Factories |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12934-019-1194-x |