Comparison of rRNA depletion methods for efficient bacterial mRNA sequencing

Abstract Current methods of high-throughput RNA sequencing of prokaryotes, including transcriptome analysis or ribosomal profiling, need deep sequencing to achieve sufficient numbers of effective reads (e.g., mapping to mRNA) in order to also find weakly expressed genetic elements. The fraction of h...

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Bibliographic Details
Main Authors: Anika Wahl, Christopher Huptas, Klaus Neuhaus
Format: Article
Language:English
Published: Nature Portfolio 2022-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-09710-y