Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements
Immunoglobulin Y is a water-soluble protein present in high concentration in hen serum and egg yolk. IgY has applications in many fields, e.g., from food stuff to the mass production of antibodies. In this work, we have implemented an electrochemical immunosensor for IgY based on templated nanoelect...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-03-01
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| Series: | Chemosensors |
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| Online Access: | http://www.mdpi.com/2227-9040/5/1/10 |
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| author | Chiara Gaetani Emmanuele Ambrosi Paolo Ugo Ligia M. Moretto |
| author_facet | Chiara Gaetani Emmanuele Ambrosi Paolo Ugo Ligia M. Moretto |
| author_sort | Chiara Gaetani |
| collection | DOAJ |
| description | Immunoglobulin Y is a water-soluble protein present in high concentration in hen serum and egg yolk. IgY has applications in many fields, e.g., from food stuff to the mass production of antibodies. In this work, we have implemented an electrochemical immunosensor for IgY based on templated nanoelectrodes ensembles. IgY is captured by the templating polycarbonate and reacted with anti-IgY labeled with horseradish peroxidase. In the presence of H2O2 and methylene blue as the redox mediator, an electrocatalytic current is generated which scales with IgY concentration in the sample. After optimizing the extracting procedure, the immunosensor was applied for analysis of fresh eggs and food integrators. The data obtained with the biosensor were validated by SDS-PAGE and Western blot measurements. |
| first_indexed | 2024-12-12T23:19:29Z |
| format | Article |
| id | doaj.art-d86dd5a99fdd43939fec620c02521f94 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-12-12T23:19:29Z |
| publishDate | 2017-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-d86dd5a99fdd43939fec620c02521f942022-12-22T00:08:19ZengMDPI AGChemosensors2227-90402017-03-01511010.3390/chemosensors5010010chemosensors5010010Electrochemical Immunosensor for Detection of IgY in Food and Food SupplementsChiara Gaetani0Emmanuele Ambrosi1Paolo Ugo2Ligia M. Moretto3Department of Molecular Sciences and Nanosystems, University Ca’ Foscari of Venice, 30174 Venice, ItalyDepartment of Molecular Sciences and Nanosystems, University Ca’ Foscari of Venice, 30174 Venice, ItalyDepartment of Molecular Sciences and Nanosystems, University Ca’ Foscari of Venice, 30174 Venice, ItalyDepartment of Molecular Sciences and Nanosystems, University Ca’ Foscari of Venice, 30174 Venice, ItalyImmunoglobulin Y is a water-soluble protein present in high concentration in hen serum and egg yolk. IgY has applications in many fields, e.g., from food stuff to the mass production of antibodies. In this work, we have implemented an electrochemical immunosensor for IgY based on templated nanoelectrodes ensembles. IgY is captured by the templating polycarbonate and reacted with anti-IgY labeled with horseradish peroxidase. In the presence of H2O2 and methylene blue as the redox mediator, an electrocatalytic current is generated which scales with IgY concentration in the sample. After optimizing the extracting procedure, the immunosensor was applied for analysis of fresh eggs and food integrators. The data obtained with the biosensor were validated by SDS-PAGE and Western blot measurements.http://www.mdpi.com/2227-9040/5/1/10immunoglobulin Yegg yolknanoelectrodes ensembleselectrochemical immunosensorfood supplementsSDS-PAGE |
| spellingShingle | Chiara Gaetani Emmanuele Ambrosi Paolo Ugo Ligia M. Moretto Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements Chemosensors immunoglobulin Y egg yolk nanoelectrodes ensembles electrochemical immunosensor food supplements SDS-PAGE |
| title | Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements |
| title_full | Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements |
| title_fullStr | Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements |
| title_full_unstemmed | Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements |
| title_short | Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements |
| title_sort | electrochemical immunosensor for detection of igy in food and food supplements |
| topic | immunoglobulin Y egg yolk nanoelectrodes ensembles electrochemical immunosensor food supplements SDS-PAGE |
| url | http://www.mdpi.com/2227-9040/5/1/10 |
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