Effects of HMGB1/TLR4 on secretion IL-10 and VEGF in human jaw bone-marrow mesenchymal stem cells
Abstract Objective: We aimed to investigate the regulatory effects of HMGB1/TLR4 signaling pathway on the expression of IL-10 and VEGF in human bone marrow mesenchymal stem cells. Methodology: Human JBMSCs were isolated and cultured. Then, HMGB1 was added into the JBMSCs culture medium, and the pr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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University of São Paulo
2024-02-01
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| Series: | Journal of Applied Oral Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572024000100407&tlng=en |
| _version_ | 1797302370922659840 |
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| author | Jingjing Kong Wei Cheng Lianzhen Chang Jingyi Yu Ronglin Wang Jianli Xie |
| author_facet | Jingjing Kong Wei Cheng Lianzhen Chang Jingyi Yu Ronglin Wang Jianli Xie |
| author_sort | Jingjing Kong |
| collection | DOAJ |
| description | Abstract Objective: We aimed to investigate the regulatory effects of HMGB1/TLR4 signaling pathway on the expression of IL-10 and VEGF in human bone marrow mesenchymal stem cells. Methodology: Human JBMSCs were isolated and cultured. Then, HMGB1 was added into the JBMSCs culture medium, and the protein and mRNA expression levels of IL-10 and VEGF were assessed. Moreover, cells were pretreated with a specific TLR4 inhibitor (TAK-242), and the expression changes of IL-10 and VEGF were compared. Results: Compared with the control group, exposure to HMGB1 in human JBMSCs up-regulated TLR4, IL-10, and VEGF secretion at both protein and mRNA levels (P<0. 05). In addition, the increased expression of IL-10 and VEGF could be restrained in TAK-242 group compared with the HMGB1 group (P<0.05). Conclusions: The results indicated that HMGB1 activate TLR4 signaling pathway in Human JBMSCs, which plays a regulatory role in cytokines expression. |
| first_indexed | 2024-03-07T23:35:52Z |
| format | Article |
| id | doaj.art-d8728b3175414fefae5a7deec8f78ff8 |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-03-07T23:35:52Z |
| publishDate | 2024-02-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-d8728b3175414fefae5a7deec8f78ff82024-02-20T07:37:06ZengUniversity of São PauloJournal of Applied Oral Science1678-77652024-02-013210.1590/1678-7757-2023-0304Effects of HMGB1/TLR4 on secretion IL-10 and VEGF in human jaw bone-marrow mesenchymal stem cellsJingjing Konghttps://orcid.org/0000-0003-3370-9756Wei ChengLianzhen ChangJingyi YuRonglin WangJianli XieAbstract Objective: We aimed to investigate the regulatory effects of HMGB1/TLR4 signaling pathway on the expression of IL-10 and VEGF in human bone marrow mesenchymal stem cells. Methodology: Human JBMSCs were isolated and cultured. Then, HMGB1 was added into the JBMSCs culture medium, and the protein and mRNA expression levels of IL-10 and VEGF were assessed. Moreover, cells were pretreated with a specific TLR4 inhibitor (TAK-242), and the expression changes of IL-10 and VEGF were compared. Results: Compared with the control group, exposure to HMGB1 in human JBMSCs up-regulated TLR4, IL-10, and VEGF secretion at both protein and mRNA levels (P<0. 05). In addition, the increased expression of IL-10 and VEGF could be restrained in TAK-242 group compared with the HMGB1 group (P<0.05). Conclusions: The results indicated that HMGB1 activate TLR4 signaling pathway in Human JBMSCs, which plays a regulatory role in cytokines expression.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572024000100407&tlng=enHMGB1JBMSCsTLR4IL-10VEGFPeri-implantitis |
| spellingShingle | Jingjing Kong Wei Cheng Lianzhen Chang Jingyi Yu Ronglin Wang Jianli Xie Effects of HMGB1/TLR4 on secretion IL-10 and VEGF in human jaw bone-marrow mesenchymal stem cells Journal of Applied Oral Science HMGB1 JBMSCs TLR4 IL-10 VEGF Peri-implantitis |
| title | Effects of HMGB1/TLR4 on secretion IL-10 and VEGF in human jaw bone-marrow mesenchymal stem cells |
| title_full | Effects of HMGB1/TLR4 on secretion IL-10 and VEGF in human jaw bone-marrow mesenchymal stem cells |
| title_fullStr | Effects of HMGB1/TLR4 on secretion IL-10 and VEGF in human jaw bone-marrow mesenchymal stem cells |
| title_full_unstemmed | Effects of HMGB1/TLR4 on secretion IL-10 and VEGF in human jaw bone-marrow mesenchymal stem cells |
| title_short | Effects of HMGB1/TLR4 on secretion IL-10 and VEGF in human jaw bone-marrow mesenchymal stem cells |
| title_sort | effects of hmgb1 tlr4 on secretion il 10 and vegf in human jaw bone marrow mesenchymal stem cells |
| topic | HMGB1 JBMSCs TLR4 IL-10 VEGF Peri-implantitis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572024000100407&tlng=en |
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