Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental <i>Aspergillus</i> Infection in Poultry
Naturally occurring human antibodies (Abs) of the isotypes IgM and IgG and reactive to the galactose-α-1,3-galactose (α-Gal) epitope are associated with protection against infectious diseases, caused by pathogens expressing the glycan. Gut microbiota bacteria expressing α-Gal regulate the immune res...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/8/2/285 |
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| author | Lourdes Mateos-Hernández Veronica Risco-Castillo Edgar Torres-Maravilla Luis G. Bermúdez-Humarán Pilar Alberdi Adnan Hodžić Angelica Hernández-Jarguin Sabine Rakotobe Clemence Galon Elodie Devillers Jose de la Fuente Jacques Guillot Alejandro Cabezas-Cruz |
| author_facet | Lourdes Mateos-Hernández Veronica Risco-Castillo Edgar Torres-Maravilla Luis G. Bermúdez-Humarán Pilar Alberdi Adnan Hodžić Angelica Hernández-Jarguin Sabine Rakotobe Clemence Galon Elodie Devillers Jose de la Fuente Jacques Guillot Alejandro Cabezas-Cruz |
| author_sort | Lourdes Mateos-Hernández |
| collection | DOAJ |
| description | Naturally occurring human antibodies (Abs) of the isotypes IgM and IgG and reactive to the galactose-α-1,3-galactose (α-Gal) epitope are associated with protection against infectious diseases, caused by pathogens expressing the glycan. Gut microbiota bacteria expressing α-Gal regulate the immune response to this glycan in animals lacking endogenous α-Gal. Here, we asked whether the production of anti-α-Gal Abs in response to microbiota stimulation in birds, confers protection against infection by <i>Aspergillus fumigatus</i>, a major fungal pathogen that expresses α-Gal in its surface. We demonstrated that the oral administration of <i>Escherichia coli</i> O86:B7 strain, a bacterium with high α-Gal content, reduces the occurrence of granulomas in lungs and protects turkeys from developing acute aspergillosis. Surprisingly, the protective effect of <i>E. coli</i> O86:B7 was not associated with an increase in circulating anti-α-Gal IgY levels, but with a striking reduction of anti-α-Gal IgA in the lungs of infected turkeys. Subcutaneous immunization against α-Gal did not induce a significant reduction of lung anti-α-Gal IgA and failed to protect against an infectious challenge with <i>A. fumigatus</i>. Oral administration of <i>E. coli</i> O86:B7 was not associated with the upregulation of lung cytokines upon <i>A. fumigatus</i> infection. We concluded that the oral administration of bacteria expressing high levels of α-Gal decreases the levels of lung anti-α-Gal IgA, which are mediators of inflammation and lung damage during acute aspergillosis. |
| first_indexed | 2024-03-10T19:20:18Z |
| format | Article |
| id | doaj.art-d881a47c0c8744258dafc3e24f7452b4 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T19:20:18Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-d881a47c0c8744258dafc3e24f7452b42023-11-20T03:06:05ZengMDPI AGVaccines2076-393X2020-06-018228510.3390/vaccines8020285Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental <i>Aspergillus</i> Infection in PoultryLourdes Mateos-Hernández0Veronica Risco-Castillo1Edgar Torres-Maravilla2Luis G. Bermúdez-Humarán3Pilar Alberdi4Adnan Hodžić5Angelica Hernández-Jarguin6Sabine Rakotobe7Clemence Galon8Elodie Devillers9Jose de la Fuente10Jacques Guillot11Alejandro Cabezas-Cruz12UMR BIPAR, INRAE, ANSES, Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est, 14 rue Pierre et Marie Curie, 94706 Maisons-Alfort, FranceEA 7380 Dynamyc, UPEC, USC, ANSES, Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est, 94700 Maisons-Alfort, FranceMicalis Institute, AgroParisTech, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMicalis Institute, AgroParisTech, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceSaBio, Instituto de Investigación en Recursos Cinegéticos (IREC-CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13005 Ciudad Real, SpainInstitute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, 1210 Vienna, AustriaSaBio, Instituto de Investigación en Recursos Cinegéticos (IREC-CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13005 Ciudad Real, SpainUMR BIPAR, INRAE, ANSES, Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est, 14 rue Pierre et Marie Curie, 94706 Maisons-Alfort, FranceUMR BIPAR, INRAE, ANSES, Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est, 14 rue Pierre et Marie Curie, 94706 Maisons-Alfort, FranceUMR BIPAR, INRAE, ANSES, Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est, 14 rue Pierre et Marie Curie, 94706 Maisons-Alfort, FranceSaBio, Instituto de Investigación en Recursos Cinegéticos (IREC-CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13005 Ciudad Real, SpainEA 7380 Dynamyc, UPEC, USC, ANSES, Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est, 94700 Maisons-Alfort, FranceUMR BIPAR, INRAE, ANSES, Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est, 14 rue Pierre et Marie Curie, 94706 Maisons-Alfort, FranceNaturally occurring human antibodies (Abs) of the isotypes IgM and IgG and reactive to the galactose-α-1,3-galactose (α-Gal) epitope are associated with protection against infectious diseases, caused by pathogens expressing the glycan. Gut microbiota bacteria expressing α-Gal regulate the immune response to this glycan in animals lacking endogenous α-Gal. Here, we asked whether the production of anti-α-Gal Abs in response to microbiota stimulation in birds, confers protection against infection by <i>Aspergillus fumigatus</i>, a major fungal pathogen that expresses α-Gal in its surface. We demonstrated that the oral administration of <i>Escherichia coli</i> O86:B7 strain, a bacterium with high α-Gal content, reduces the occurrence of granulomas in lungs and protects turkeys from developing acute aspergillosis. Surprisingly, the protective effect of <i>E. coli</i> O86:B7 was not associated with an increase in circulating anti-α-Gal IgY levels, but with a striking reduction of anti-α-Gal IgA in the lungs of infected turkeys. Subcutaneous immunization against α-Gal did not induce a significant reduction of lung anti-α-Gal IgA and failed to protect against an infectious challenge with <i>A. fumigatus</i>. Oral administration of <i>E. coli</i> O86:B7 was not associated with the upregulation of lung cytokines upon <i>A. fumigatus</i> infection. We concluded that the oral administration of bacteria expressing high levels of α-Gal decreases the levels of lung anti-α-Gal IgA, which are mediators of inflammation and lung damage during acute aspergillosis.https://www.mdpi.com/2076-393X/8/2/285alpha-Galmicrobiotaaspergillosis<i>Aspergillus fumigatus</i>granulomascross-protective immunity |
| spellingShingle | Lourdes Mateos-Hernández Veronica Risco-Castillo Edgar Torres-Maravilla Luis G. Bermúdez-Humarán Pilar Alberdi Adnan Hodžić Angelica Hernández-Jarguin Sabine Rakotobe Clemence Galon Elodie Devillers Jose de la Fuente Jacques Guillot Alejandro Cabezas-Cruz Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental <i>Aspergillus</i> Infection in Poultry Vaccines alpha-Gal microbiota aspergillosis <i>Aspergillus fumigatus</i> granulomas cross-protective immunity |
| title | Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental <i>Aspergillus</i> Infection in Poultry |
| title_full | Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental <i>Aspergillus</i> Infection in Poultry |
| title_fullStr | Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental <i>Aspergillus</i> Infection in Poultry |
| title_full_unstemmed | Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental <i>Aspergillus</i> Infection in Poultry |
| title_short | Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental <i>Aspergillus</i> Infection in Poultry |
| title_sort | gut microbiota abrogates anti α gal iga response in lungs and protects against experimental i aspergillus i infection in poultry |
| topic | alpha-Gal microbiota aspergillosis <i>Aspergillus fumigatus</i> granulomas cross-protective immunity |
| url | https://www.mdpi.com/2076-393X/8/2/285 |
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