Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma

Fluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds. An increase in fluorescence intensity was dependent on IgG...

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Bibliographic Details
Main Authors: Kiyotaka Sakai, Takehito Ogawa, Takehiro Miyasaka, Satoka Aoyagi
Format: Article
Language:English
Published: MDPI AG 2009-10-01
Series:Sensors
Subjects:
Online Access:http://www.mdpi.com/1424-8220/9/10/8271/