Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma
Fluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds. An increase in fluorescence intensity was dependent on IgG...
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| Format: | Article |
| Language: | English |
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MDPI AG
2009-10-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/9/10/8271/ |
| _version_ | 1798032182016475136 |
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| author | Kiyotaka Sakai Takehito Ogawa Takehiro Miyasaka Satoka Aoyagi |
| author_facet | Kiyotaka Sakai Takehito Ogawa Takehiro Miyasaka Satoka Aoyagi |
| author_sort | Kiyotaka Sakai |
| collection | DOAJ |
| description | Fluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds. An increase in fluorescence intensity was dependent on IgG concentration ranging from 20 to 78 μg/mL in both phosphate buffer saline and bovine plasma. This method requires no separation procedure, and the reaction time is less than 15 min. A fluorescence enhancement assay by the affinity binding of fluorescence-labeled reagent is thus available for the rapid determination of biomolecules in plasma. |
| first_indexed | 2024-04-11T20:09:45Z |
| format | Article |
| id | doaj.art-d88d18ab0ea445cba66f78f10c93d45c |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T20:09:45Z |
| publishDate | 2009-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-d88d18ab0ea445cba66f78f10c93d45c2022-12-22T04:05:12ZengMDPI AGSensors1424-82202009-10-019108271827710.3390/s91008271Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine PlasmaKiyotaka SakaiTakehito OgawaTakehiro MiyasakaSatoka AoyagiFluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds. An increase in fluorescence intensity was dependent on IgG concentration ranging from 20 to 78 μg/mL in both phosphate buffer saline and bovine plasma. This method requires no separation procedure, and the reaction time is less than 15 min. A fluorescence enhancement assay by the affinity binding of fluorescence-labeled reagent is thus available for the rapid determination of biomolecules in plasma.http://www.mdpi.com/1424-8220/9/10/8271/fluorescence enhancementimmunoassayimmunoglobulin GProtein A |
| spellingShingle | Kiyotaka Sakai Takehito Ogawa Takehiro Miyasaka Satoka Aoyagi Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma Sensors fluorescence enhancement immunoassay immunoglobulin G Protein A |
| title | Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma |
| title_full | Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma |
| title_fullStr | Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma |
| title_full_unstemmed | Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma |
| title_short | Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma |
| title_sort | fluorescence enhancement of fluorescein isothiocyanate labeled protein a caused by affinity binding with immunoglobulin g in bovine plasma |
| topic | fluorescence enhancement immunoassay immunoglobulin G Protein A |
| url | http://www.mdpi.com/1424-8220/9/10/8271/ |
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