Data of transcriptional effects of the merbarone-mediated inhibition of TOP2
Type II DNA topoisomerases relax topological stress by transiently gating DNA passage in a controlled cut-and-reseal mechanism that affects both DNA strands. Therefore, they are essential to overcome topological problems associated with DNA metabolism. Their aberrant activity results in the generati...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Data in Brief |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S235234092200693X |
| _version_ | 1828145056871088128 |
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| author | Fernando M. Delgado-Chaves Pedro Manuel Martínez-García Andrés Herrero-Ruiz Francisco Gómez-Vela Federico Divina Silvia Jimeno-González Felipe Cortés-Ledesma |
| author_facet | Fernando M. Delgado-Chaves Pedro Manuel Martínez-García Andrés Herrero-Ruiz Francisco Gómez-Vela Federico Divina Silvia Jimeno-González Felipe Cortés-Ledesma |
| author_sort | Fernando M. Delgado-Chaves |
| collection | DOAJ |
| description | Type II DNA topoisomerases relax topological stress by transiently gating DNA passage in a controlled cut-and-reseal mechanism that affects both DNA strands. Therefore, they are essential to overcome topological problems associated with DNA metabolism. Their aberrant activity results in the generation of DNA double-strand breaks, which can seriously compromise cell survival and genome integrity. Here, we profile the transcriptome of human-telomerase-immortalized retinal pigment epithelial 1 (RPE-1) cells when treated with merbarone, a drug that catalytically inhibits type II DNA topoisomerases. We performed RNA-Seq after 4 and 8 h of merbarone treatment and compared transcriptional profiles versus untreated samples. We report raw sequencing data together with lists of gene counts and differentially expressed genes. |
| first_indexed | 2024-04-11T20:26:29Z |
| format | Article |
| id | doaj.art-d89eac8543cc469fb29754a4a04edb84 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-04-11T20:26:29Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-d89eac8543cc469fb29754a4a04edb842022-12-22T04:04:38ZengElsevierData in Brief2352-34092022-10-0144108499Data of transcriptional effects of the merbarone-mediated inhibition of TOP2Fernando M. Delgado-Chaves0Pedro Manuel Martínez-García1Andrés Herrero-Ruiz2Francisco Gómez-Vela3Federico Divina4Silvia Jimeno-González5Felipe Cortés-Ledesma6Division of Computer Science, Pablo de Olavide University, Seville ES-41013, Spain; Corresponding authors.Andalusian Molecular Biology and Regenerative Medicine Center (CABIMER), University of Seville - CSIC - Pablo de Olavide University, Seville ES-41092 SpainAndalusian Molecular Biology and Regenerative Medicine Center (CABIMER), University of Seville - CSIC - Pablo de Olavide University, Seville ES-41092 Spain; Topology and DNA breaks Group, Spanish National Cancer Research Center (CNIO), Madrid ES-28029, SpainDivision of Computer Science, Pablo de Olavide University, Seville ES-41013, SpainDivision of Computer Science, Pablo de Olavide University, Seville ES-41013, SpainAndalusian Molecular Biology and Regenerative Medicine Center (CABIMER), University of Seville - CSIC - Pablo de Olavide University, Seville ES-41092 SpainTopology and DNA breaks Group, Spanish National Cancer Research Center (CNIO), Madrid ES-28029, Spain; Corresponding authors.Type II DNA topoisomerases relax topological stress by transiently gating DNA passage in a controlled cut-and-reseal mechanism that affects both DNA strands. Therefore, they are essential to overcome topological problems associated with DNA metabolism. Their aberrant activity results in the generation of DNA double-strand breaks, which can seriously compromise cell survival and genome integrity. Here, we profile the transcriptome of human-telomerase-immortalized retinal pigment epithelial 1 (RPE-1) cells when treated with merbarone, a drug that catalytically inhibits type II DNA topoisomerases. We performed RNA-Seq after 4 and 8 h of merbarone treatment and compared transcriptional profiles versus untreated samples. We report raw sequencing data together with lists of gene counts and differentially expressed genes.http://www.sciencedirect.com/science/article/pii/S235234092200693XTopoisomerase inhibitionDifferential gene expressionDNA supercoilingMerbaroneRNA-Seq |
| spellingShingle | Fernando M. Delgado-Chaves Pedro Manuel Martínez-García Andrés Herrero-Ruiz Francisco Gómez-Vela Federico Divina Silvia Jimeno-González Felipe Cortés-Ledesma Data of transcriptional effects of the merbarone-mediated inhibition of TOP2 Data in Brief Topoisomerase inhibition Differential gene expression DNA supercoiling Merbarone RNA-Seq |
| title | Data of transcriptional effects of the merbarone-mediated inhibition of TOP2 |
| title_full | Data of transcriptional effects of the merbarone-mediated inhibition of TOP2 |
| title_fullStr | Data of transcriptional effects of the merbarone-mediated inhibition of TOP2 |
| title_full_unstemmed | Data of transcriptional effects of the merbarone-mediated inhibition of TOP2 |
| title_short | Data of transcriptional effects of the merbarone-mediated inhibition of TOP2 |
| title_sort | data of transcriptional effects of the merbarone mediated inhibition of top2 |
| topic | Topoisomerase inhibition Differential gene expression DNA supercoiling Merbarone RNA-Seq |
| url | http://www.sciencedirect.com/science/article/pii/S235234092200693X |
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