Uniform design methods for high efficient micropropagation of Platanthera mandarinorum Rchb. f. var. cornu-bovis (Nevski) Kitag. and Coeloglossum viride (L.) Hartm(基于均匀设计法优化长白舌唇兰和凹舌兰的高效快繁体系)
以长白舌唇兰和凹舌兰的茎尖为外植体,应用均匀设计法筛选最适合于原球茎和类原球茎诱导及类原球茎萌发为完整植株的培养基.结果表明,最适合长白舌唇兰原球茎和类原球茎诱导的培养基为N6 +TDZ 0.05 mg •L-1 + NAA0.01 mg •L-1 +KT0.50 mg •L-1,诱导率为92.5%；最适合凹舌兰原球茎和类原球茎诱导的培养基为N6 + TDZ0.05 mg •L-1 +KT0.50 mg •L-1,诱导率为98%；长白舌唇兰类原球茎萌发为完整植株的最佳培养基为N6+IAA0.01 mg •L-1,萌发率为96%；凹舌兰类原球茎萌发为完整植株的最佳培养基为N6 + IAA0.01...
| Main Authors: | , |
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| Format: | Article |
| Language: | zho |
| Published: |
Zhejiang University Press
2010-03-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2010.02.021 |