Uniform design methods for high efficient micropropagation of Platanthera mandarinorum Rchb. f. var. cornu-bovis (Nevski) Kitag. and Coeloglossum viride (L.) Hartm(基于均匀设计法优化长白舌唇兰和凹舌兰的高效快繁体系)
以长白舌唇兰和凹舌兰的茎尖为外植体,应用均匀设计法筛选最适合于原球茎和类原球茎诱导及类原球茎萌发为完整植株的培养基.结果表明,最适合长白舌唇兰原球茎和类原球茎诱导的培养基为N6 +TDZ 0.05 mg •L-1 + NAA0.01 mg •L-1 +KT0.50 mg •L-1,诱导率为92.5%；最适合凹舌兰原球茎和类原球茎诱导的培养基为N6 + TDZ0.05 mg •L-1 +KT0.50 mg •L-1,诱导率为98%；长白舌唇兰类原球茎萌发为完整植株的最佳培养基为N6+IAA0.01 mg •L-1,萌发率为96%；凹舌兰类原球茎萌发为完整植株的最佳培养基为N6 + IAA0.01...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Zhejiang University Press
2010-03-01
|
| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2010.02.021 |
| _version_ | 1797236192373112832 |
|---|---|
| author | GUDi-zhou(顾地周) GAOHan-dong(高捍东) |
| author_facet | GUDi-zhou(顾地周) GAOHan-dong(高捍东) |
| author_sort | GUDi-zhou(顾地周) |
| collection | DOAJ |
| description | 以长白舌唇兰和凹舌兰的茎尖为外植体,应用均匀设计法筛选最适合于原球茎和类原球茎诱导及类原球茎萌发为完整植株的培养基.结果表明,最适合长白舌唇兰原球茎和类原球茎诱导的培养基为N6 +TDZ 0.05 mg •L-1 + NAA0.01 mg •L-1 +KT0.50 mg •L-1,诱导率为92.5%；最适合凹舌兰原球茎和类原球茎诱导的培养基为N6 + TDZ0.05 mg •L-1 +KT0.50 mg •L-1,诱导率为98%；长白舌唇兰类原球茎萌发为完整植株的最佳培养基为N6+IAA0.01 mg •L-1,萌发率为96%；凹舌兰类原球茎萌发为完整植株的最佳培养基为N6 + IAA0.01 mg •L-1 +GA30.01 mg •L-1,萌发率为94.5%.以类原球茎的切片为材料进行类原球茎快繁的结果表明,在30~40 d的一个培养周期内,增殖倍数达100以上,成功建立了两种兰的高效快繁体系.同时对不同阶段培养材料的形态结构及超微结构的观察证明了两种兰的类原球茎发生发育过程. |
| first_indexed | 2024-04-24T16:59:57Z |
| format | Article |
| id | doaj.art-d8b6822cf1804ab2898265499ba58b46 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T16:59:57Z |
| publishDate | 2010-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-d8b6822cf1804ab2898265499ba58b462024-03-29T01:58:28ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972010-03-0137222122610.3785/j.issn.1008-9497.2010.02.021Uniform design methods for high efficient micropropagation of Platanthera mandarinorum Rchb. f. var. cornu-bovis (Nevski) Kitag. and Coeloglossum viride (L.) Hartm(基于均匀设计法优化长白舌唇兰和凹舌兰的高效快繁体系)GUDi-zhou(顾地周)0GAOHan-dong(高捍东)1 1.Department of Biology, Tonghua Normal University, Tonghua 134002, Jilin Province, China( 1.通化师范学院生物系，吉林 通化 134002) 2.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China( 2.南京林业大学森林资源与环境学院，江苏 南京 210037)以长白舌唇兰和凹舌兰的茎尖为外植体,应用均匀设计法筛选最适合于原球茎和类原球茎诱导及类原球茎萌发为完整植株的培养基.结果表明,最适合长白舌唇兰原球茎和类原球茎诱导的培养基为N6 +TDZ 0.05 mg •L-1 + NAA0.01 mg •L-1 +KT0.50 mg •L-1,诱导率为92.5%；最适合凹舌兰原球茎和类原球茎诱导的培养基为N6 + TDZ0.05 mg •L-1 +KT0.50 mg •L-1,诱导率为98%；长白舌唇兰类原球茎萌发为完整植株的最佳培养基为N6+IAA0.01 mg •L-1,萌发率为96%；凹舌兰类原球茎萌发为完整植株的最佳培养基为N6 + IAA0.01 mg •L-1 +GA30.01 mg •L-1,萌发率为94.5%.以类原球茎的切片为材料进行类原球茎快繁的结果表明,在30~40 d的一个培养周期内,增殖倍数达100以上,成功建立了两种兰的高效快繁体系.同时对不同阶段培养材料的形态结构及超微结构的观察证明了两种兰的类原球茎发生发育过程.https://doi.org/10.3785/j.issn.1008-9497.2010.02.021长白舌唇兰凹舌兰均匀设计原球茎类原球茎 |
| spellingShingle | GUDi-zhou(顾地周) GAOHan-dong(高捍东) Uniform design methods for high efficient micropropagation of Platanthera mandarinorum Rchb. f. var. cornu-bovis (Nevski) Kitag. and Coeloglossum viride (L.) Hartm(基于均匀设计法优化长白舌唇兰和凹舌兰的高效快繁体系) Zhejiang Daxue xuebao. Lixue ban 长白舌唇兰 凹舌兰 均匀设计 原球茎 类原球茎 |
| title | Uniform design methods for high efficient micropropagation of Platanthera mandarinorum Rchb. f. var. cornu-bovis (Nevski) Kitag. and Coeloglossum viride (L.) Hartm(基于均匀设计法优化长白舌唇兰和凹舌兰的高效快繁体系) |
| title_full | Uniform design methods for high efficient micropropagation of Platanthera mandarinorum Rchb. f. var. cornu-bovis (Nevski) Kitag. and Coeloglossum viride (L.) Hartm(基于均匀设计法优化长白舌唇兰和凹舌兰的高效快繁体系) |
| title_fullStr | Uniform design methods for high efficient micropropagation of Platanthera mandarinorum Rchb. f. var. cornu-bovis (Nevski) Kitag. and Coeloglossum viride (L.) Hartm(基于均匀设计法优化长白舌唇兰和凹舌兰的高效快繁体系) |
| title_full_unstemmed | Uniform design methods for high efficient micropropagation of Platanthera mandarinorum Rchb. f. var. cornu-bovis (Nevski) Kitag. and Coeloglossum viride (L.) Hartm(基于均匀设计法优化长白舌唇兰和凹舌兰的高效快繁体系) |
| title_short | Uniform design methods for high efficient micropropagation of Platanthera mandarinorum Rchb. f. var. cornu-bovis (Nevski) Kitag. and Coeloglossum viride (L.) Hartm(基于均匀设计法优化长白舌唇兰和凹舌兰的高效快繁体系) |
| title_sort | uniform design methods for high efficient micropropagation of platanthera mandarinorum rchb f var cornu bovis nevski kitag and coeloglossum viride l hartm 基于均匀设计法优化长白舌唇兰和凹舌兰的高效快繁体系 |
| topic | 长白舌唇兰 凹舌兰 均匀设计 原球茎 类原球茎 |
| url | https://doi.org/10.3785/j.issn.1008-9497.2010.02.021 |
| work_keys_str_mv | AT gudizhougùdezhōu uniformdesignmethodsforhighefficientmicropropagationofplatantheramandarinorumrchbfvarcornubovisnevskikitagandcoeloglossumviridelhartmjīyújūnyúnshèjìfǎyōuhuàzhǎngbáishéchúnlánhéāoshélándegāoxiàokuàifántǐxì AT gaohandonggāohàndōng uniformdesignmethodsforhighefficientmicropropagationofplatantheramandarinorumrchbfvarcornubovisnevskikitagandcoeloglossumviridelhartmjīyújūnyúnshèjìfǎyōuhuàzhǎngbáishéchúnlánhéāoshélándegāoxiàokuàifántǐxì |