Identification and quantification of viable Lacticaseibacillus rhamnosus in probiotics using validated PMA-qPCR method

Identification and quantification of viable Lacticaseibacillus rhamnosus in probiotics using validated PMA-qPCR method

The identification and quantification of viable bacteria at the species/strain level in compound probiotic products is challenging now. Molecular biology methods, e.g., propidium monoazide (PMA) combination with qPCR, have gained prominence for targeted viable cell counts. This study endeavors to es...

Full description

Bibliographic Details
Main Authors: Lizheng Guo, Xiaolei Ze, Huifen Feng, Yiru Liu, Yuanyuan Ge, Xi Zhao, Chengyu Song, Yingxin Jiao, Jiaqi Liu, Shuaicheng Mu, Su Yao
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-01-01
Series:Frontiers in Microbiology
Subjects:
probiotics
Lacticaseibacillus rhamnosus
identification
viable cell quantification
PMA-qPCR method
validation
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2024.1341884/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2024.1341884/full

Similar Items