Evaluation of Basidiomycetes Wild Strains Grown in Agro-Industrial Residues for Their Anti-Tyrosinase and Antioxidant Potential and for the Production of Biocatalysts
White-rot basidiomycetes are the only microorganisms with the ability to produce both hydrolytic (cellulases and hemicellulases) and oxidative (ligninolytic) enzymes for degrading cellulose/hemicellulose and lignin. In addition, they produce biologically active natural products with important applic...
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MDPI AG
2021-02-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/7/1/19 |
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| author | Anastasia Zerva Nikolaos Tsafantakis Evangelos Topakas |
| author_facet | Anastasia Zerva Nikolaos Tsafantakis Evangelos Topakas |
| author_sort | Anastasia Zerva |
| collection | DOAJ |
| description | White-rot basidiomycetes are the only microorganisms with the ability to produce both hydrolytic (cellulases and hemicellulases) and oxidative (ligninolytic) enzymes for degrading cellulose/hemicellulose and lignin. In addition, they produce biologically active natural products with important application in cosmetic formulations, either as pure compounds or as standardized extracts. In the present work, three wild strains of Basidiomycetes fungi (<i>Pleurotus citrinopileatus</i>, <i>Abortiporus biennis</i> and <i>Ganoderma resinaceum</i>) from Greek habitats were grown in agro-industrial residues (oil mill wastewater, and corn cob) and evaluated for their anti-tyrosinase and antioxidant activity and for the production of biotechnologically relevant enzymes. <i>P. citrinopileatus</i> showed the most interesting tyrosinase inhibitory activity, while <i>A. biennis</i> showed the highest DPPH(2,2-diphenyl-1-picryl-hydrazyl) scavenging potential. Corn cobs were the most appropriate carbon source for maximizing the inhibitory effect of fungal biomasses on both activities, while the use of oil mill wastewater selectively increased the anti-tyrosinase potential of <i>P. citrinopileatus</i> culture filtrate. All strains were found to be preferential lignin degraders, similarly to most white-rot fungi. Bioinformatic analyses were performed on the proteome of the strains <i>P. citrinopileatus</i> and <i>A. biennis</i>, focusing on CAZymes with biotechnological relevance, and the results were compared with the enzyme activities of culture supernatants. Overall, all three strains showed strong production of oxidative enzymes for biomass conversion applications. |
| first_indexed | 2024-03-09T06:11:59Z |
| format | Article |
| id | doaj.art-d8c5d13f6877439490ab40a17e1cea4e |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T06:11:59Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-d8c5d13f6877439490ab40a17e1cea4e2023-12-03T11:57:58ZengMDPI AGFermentation2311-56372021-02-01711910.3390/fermentation7010019Evaluation of Basidiomycetes Wild Strains Grown in Agro-Industrial Residues for Their Anti-Tyrosinase and Antioxidant Potential and for the Production of BiocatalystsAnastasia Zerva0Nikolaos Tsafantakis1Evangelos Topakas2Industrial Biotechnology & Biocatalysis Group, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str., Zografou Campus, 15780 Athens, GreeceDepartment of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, GreeceIndustrial Biotechnology & Biocatalysis Group, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str., Zografou Campus, 15780 Athens, GreeceWhite-rot basidiomycetes are the only microorganisms with the ability to produce both hydrolytic (cellulases and hemicellulases) and oxidative (ligninolytic) enzymes for degrading cellulose/hemicellulose and lignin. In addition, they produce biologically active natural products with important application in cosmetic formulations, either as pure compounds or as standardized extracts. In the present work, three wild strains of Basidiomycetes fungi (<i>Pleurotus citrinopileatus</i>, <i>Abortiporus biennis</i> and <i>Ganoderma resinaceum</i>) from Greek habitats were grown in agro-industrial residues (oil mill wastewater, and corn cob) and evaluated for their anti-tyrosinase and antioxidant activity and for the production of biotechnologically relevant enzymes. <i>P. citrinopileatus</i> showed the most interesting tyrosinase inhibitory activity, while <i>A. biennis</i> showed the highest DPPH(2,2-diphenyl-1-picryl-hydrazyl) scavenging potential. Corn cobs were the most appropriate carbon source for maximizing the inhibitory effect of fungal biomasses on both activities, while the use of oil mill wastewater selectively increased the anti-tyrosinase potential of <i>P. citrinopileatus</i> culture filtrate. All strains were found to be preferential lignin degraders, similarly to most white-rot fungi. Bioinformatic analyses were performed on the proteome of the strains <i>P. citrinopileatus</i> and <i>A. biennis</i>, focusing on CAZymes with biotechnological relevance, and the results were compared with the enzyme activities of culture supernatants. Overall, all three strains showed strong production of oxidative enzymes for biomass conversion applications.https://www.mdpi.com/2311-5637/7/1/19basidiomycetesnovel enzymesbiocatalystsanti-tyrosinase activityantioxidant activitylaccases |
| spellingShingle | Anastasia Zerva Nikolaos Tsafantakis Evangelos Topakas Evaluation of Basidiomycetes Wild Strains Grown in Agro-Industrial Residues for Their Anti-Tyrosinase and Antioxidant Potential and for the Production of Biocatalysts Fermentation basidiomycetes novel enzymes biocatalysts anti-tyrosinase activity antioxidant activity laccases |
| title | Evaluation of Basidiomycetes Wild Strains Grown in Agro-Industrial Residues for Their Anti-Tyrosinase and Antioxidant Potential and for the Production of Biocatalysts |
| title_full | Evaluation of Basidiomycetes Wild Strains Grown in Agro-Industrial Residues for Their Anti-Tyrosinase and Antioxidant Potential and for the Production of Biocatalysts |
| title_fullStr | Evaluation of Basidiomycetes Wild Strains Grown in Agro-Industrial Residues for Their Anti-Tyrosinase and Antioxidant Potential and for the Production of Biocatalysts |
| title_full_unstemmed | Evaluation of Basidiomycetes Wild Strains Grown in Agro-Industrial Residues for Their Anti-Tyrosinase and Antioxidant Potential and for the Production of Biocatalysts |
| title_short | Evaluation of Basidiomycetes Wild Strains Grown in Agro-Industrial Residues for Their Anti-Tyrosinase and Antioxidant Potential and for the Production of Biocatalysts |
| title_sort | evaluation of basidiomycetes wild strains grown in agro industrial residues for their anti tyrosinase and antioxidant potential and for the production of biocatalysts |
| topic | basidiomycetes novel enzymes biocatalysts anti-tyrosinase activity antioxidant activity laccases |
| url | https://www.mdpi.com/2311-5637/7/1/19 |
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