Protein purification, crystallization, and structure determination of transcription factor YhaJ in complex with DNT metabolites
Summary: The microbial transcription factor YhaJ responds to 2,4-dinitrotoluene (DNT) derivatives. Here, we describe steps for overexpression and purification of the protein, characterization for the binding of a DNT derivative methylhydroquinone, and crystallization by using a random seeding techni...
| Autori principali: | , , , |
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| Natura: | Articolo |
| Lingua: | English |
| Pubblicazione: |
Elsevier
2024-06-01
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| Serie: | STAR Protocols |
| Soggetti: | |
| Accesso online: | http://www.sciencedirect.com/science/article/pii/S2666166724001643 |