Protein purification, crystallization, and structure determination of transcription factor YhaJ in complex with DNT metabolites

Protein purification, crystallization, and structure determination of transcription factor YhaJ in complex with DNT metabolites

Summary: The microbial transcription factor YhaJ responds to 2,4-dinitrotoluene (DNT) derivatives. Here, we describe steps for overexpression and purification of the protein, characterization for the binding of a DNT derivative methylhydroquinone, and crystallization by using a random seeding techni...

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Bibliographic Details
Main Authors:	Myeongbin Kim, Ryun Kang, Tae Jin Jeon, Seong Eon Ryu
Format:	Article
Language:	English
Published:	Elsevier 2024-06-01
Series:	STAR Protocols
Subjects:	Protein expression and purification X-ray Crystallography Biotechnology and bioengineering
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724001643

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