Protocol for IPO11 deletion and re-expression in H460 lung cancer cells using CRISPR-Cas9 and plasmid transfection

Protocol for IPO11 deletion and re-expression in H460 lung cancer cells using CRISPR-Cas9 and plasmid transfection

Summary: Exploring the essential role of Importin 11 (IPO11) in the nuclear translocation of its potential cargo proteins requires an efficient means of IPO11 deletion and re-expression. Here, we present a protocol for the generation of IPO11 deletion using CRISPR-Cas9 and re-expression using plasmi...

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Bibliographic Details
Main Authors: Yarui Zhang, Guomin Ou, Zhou Zhou, Jingdong Wang, Hongying Yang
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cancer
Cell Biology
CRISPR
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723002848

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http://www.sciencedirect.com/science/article/pii/S2666166723002848

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