CRISPR-Cas9 Editing Induces Loss of Heterozygosity in the Pathogenic Yeast Candida parapsilosis

CRISPR-Cas9 Editing Induces Loss of Heterozygosity in the Pathogenic Yeast Candida parapsilosis

ABSTRACT Genetic manipulation is often used to study gene function. However, unplanned genome changes (including single nucleotide polymorphisms [SNPs], aneuploidy, and loss of heterozygosity [LOH]) can affect the phenotypic traits of the engineered strains. Here, we compared the effect of classical...

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Bibliographic Details
Main Authors: Lisa Lombardi, Sean A. Bergin, Adam Ryan, Evelyn Zuniga-Soto, Geraldine Butler
Format: Article
Language:English
Published: American Society for Microbiology 2022-12-01
Series:mSphere
Subjects:
CRISPR-Cas9
Candida parapsilosis
loss of heterozygosity
genome editing
Online Access:https://journals.asm.org/doi/10.1128/msphere.00393-22

Internet

https://journals.asm.org/doi/10.1128/msphere.00393-22

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