Further Extension of Lifespan by <i>Unc-43/CaMKII</i> and <i>Egl-8/PLCβ</i> Mutations in Germline-Deficient <i>Caenorhabditis elegans</i>
Reduction of insulin/insulin-like growth factor 1 (IGF1) signaling (IIS) promotes longevity across species. In the nematode <i>Caenorhabditis elegans</i>, ablation of germline stem cells (GSCs) and activity changes of the conserved signaling mediators <i>unc-43/CaMKII</i> (ca...
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MDPI AG
2022-11-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/22/3527 |
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| author | Hildegard I. D. Mack Laura G. Buck Sonja Skalet Jennifer Kremer Hao Li Elisabeth K. M. Mack |
| author_facet | Hildegard I. D. Mack Laura G. Buck Sonja Skalet Jennifer Kremer Hao Li Elisabeth K. M. Mack |
| author_sort | Hildegard I. D. Mack |
| collection | DOAJ |
| description | Reduction of insulin/insulin-like growth factor 1 (IGF1) signaling (IIS) promotes longevity across species. In the nematode <i>Caenorhabditis elegans</i>, ablation of germline stem cells (GSCs) and activity changes of the conserved signaling mediators <i>unc-43/CaMKII</i> (calcium/calmodulin-dependent kinase type II) and <i>egl-8/PLCβ</i> (phospholipase Cβ) also increase lifespan. Like IIS, these pathways depend on the conserved transcription factor <i>daf-16/FOXO</i> for lifespan extension, but how they functionally interact is unknown. Here, we show that altered <i>unc-43/egl-8</i> activity further increases the lifespan of long-lived GSC-deficient worms, but not of worms that are long-lived due to a strong reduction-of-function mutation in the insulin/IGF1-like receptor <i>daf-2</i>. Additionally, we provide evidence for <i>unc-43</i> and, to a lesser extent, <i>egl-8</i> modulating the expression of certain collagen genes, which were reported to be dispensable for longevity of these particular <i>daf-2</i> mutant worms, but not for other forms of longevity. Together, these results provide new insights into the conditions and potential mechanisms by which CaMKII- and PLCβ-signals modulate <i>C. elegans</i> lifespan. |
| first_indexed | 2024-03-09T18:25:49Z |
| format | Article |
| id | doaj.art-d912b26fa9b0409382be19a67f0eda6b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T18:25:49Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-d912b26fa9b0409382be19a67f0eda6b2023-11-24T07:56:53ZengMDPI AGCells2073-44092022-11-011122352710.3390/cells11223527Further Extension of Lifespan by <i>Unc-43/CaMKII</i> and <i>Egl-8/PLCβ</i> Mutations in Germline-Deficient <i>Caenorhabditis elegans</i>Hildegard I. D. Mack0Laura G. Buck1Sonja Skalet2Jennifer Kremer3Hao Li4Elisabeth K. M. Mack5Institute for Biomedical Aging Research, Leopold-Franzens-Universität Innsbruck, 6020 Innsbruck, AustriaInstitute for Biomedical Aging Research, Leopold-Franzens-Universität Innsbruck, 6020 Innsbruck, AustriaInstitute for Biomedical Aging Research, Leopold-Franzens-Universität Innsbruck, 6020 Innsbruck, AustriaDepartment of Hematology, Oncology and Immunology, Philipps-University Marburg, 35043 Marburg, GermanyDepartment of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USADepartment of Hematology, Oncology and Immunology, Philipps-University Marburg, 35043 Marburg, GermanyReduction of insulin/insulin-like growth factor 1 (IGF1) signaling (IIS) promotes longevity across species. In the nematode <i>Caenorhabditis elegans</i>, ablation of germline stem cells (GSCs) and activity changes of the conserved signaling mediators <i>unc-43/CaMKII</i> (calcium/calmodulin-dependent kinase type II) and <i>egl-8/PLCβ</i> (phospholipase Cβ) also increase lifespan. Like IIS, these pathways depend on the conserved transcription factor <i>daf-16/FOXO</i> for lifespan extension, but how they functionally interact is unknown. Here, we show that altered <i>unc-43/egl-8</i> activity further increases the lifespan of long-lived GSC-deficient worms, but not of worms that are long-lived due to a strong reduction-of-function mutation in the insulin/IGF1-like receptor <i>daf-2</i>. Additionally, we provide evidence for <i>unc-43</i> and, to a lesser extent, <i>egl-8</i> modulating the expression of certain collagen genes, which were reported to be dispensable for longevity of these particular <i>daf-2</i> mutant worms, but not for other forms of longevity. Together, these results provide new insights into the conditions and potential mechanisms by which CaMKII- and PLCβ-signals modulate <i>C. elegans</i> lifespan.https://www.mdpi.com/2073-4409/11/22/3527agingstress resistanceinsulin signalinggermline stem cellsRNA-seqcollagen |
| spellingShingle | Hildegard I. D. Mack Laura G. Buck Sonja Skalet Jennifer Kremer Hao Li Elisabeth K. M. Mack Further Extension of Lifespan by <i>Unc-43/CaMKII</i> and <i>Egl-8/PLCβ</i> Mutations in Germline-Deficient <i>Caenorhabditis elegans</i> Cells aging stress resistance insulin signaling germline stem cells RNA-seq collagen |
| title | Further Extension of Lifespan by <i>Unc-43/CaMKII</i> and <i>Egl-8/PLCβ</i> Mutations in Germline-Deficient <i>Caenorhabditis elegans</i> |
| title_full | Further Extension of Lifespan by <i>Unc-43/CaMKII</i> and <i>Egl-8/PLCβ</i> Mutations in Germline-Deficient <i>Caenorhabditis elegans</i> |
| title_fullStr | Further Extension of Lifespan by <i>Unc-43/CaMKII</i> and <i>Egl-8/PLCβ</i> Mutations in Germline-Deficient <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | Further Extension of Lifespan by <i>Unc-43/CaMKII</i> and <i>Egl-8/PLCβ</i> Mutations in Germline-Deficient <i>Caenorhabditis elegans</i> |
| title_short | Further Extension of Lifespan by <i>Unc-43/CaMKII</i> and <i>Egl-8/PLCβ</i> Mutations in Germline-Deficient <i>Caenorhabditis elegans</i> |
| title_sort | further extension of lifespan by i unc 43 camkii i and i egl 8 plcβ i mutations in germline deficient i caenorhabditis elegans i |
| topic | aging stress resistance insulin signaling germline stem cells RNA-seq collagen |
| url | https://www.mdpi.com/2073-4409/11/22/3527 |
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