Inter-genus gene expression analysis in livestock fibroblasts using reference gene validation based upon a multi-species primer set.

Inter-genus gene expression analysis in livestock fibroblasts using reference gene validation based upon a multi-species primer set.

Quantitative reverse transcription PCR (RT-qPCR) remains as an accurate approach for gene expression analysis but requires labor-intensive validation of reference genes using species-specific primers. To ease such demand, the aim was to design and test a multi-species primer set to validate referenc...

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Bibliographic Details
Main Authors: Marcelo T Moura, Roberta L O Silva, Pábola S Nascimento, José C Ferreira-Silva, Ludymila F Cantanhêde, Ederson A Kido, Ana M Benko-Iseppon, Marcos A L Oliveira
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0221170

Internet

https://doi.org/10.1371/journal.pone.0221170

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