Prospective Comparison Between Shotgun Metagenomics and Sanger Sequencing of the 16S rRNA Gene for the Etiological Diagnosis of Infections

Prospective Comparison Between Shotgun Metagenomics and Sanger Sequencing of the 16S rRNA Gene for the Etiological Diagnosis of Infections

Bacteriological diagnosis is traditionally based on culture. However, this method may be limited by the difficulty of cultivating certain species or by prior exposure to antibiotics, which justifies the resort to molecular methods, such as Sanger sequencing of the 16S rRNA gene (Sanger 16S). Recentl...

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Bibliographic Details
Main Authors: Claudie Lamoureux, Laure Surgers, Vincent Fihman, Guillaume Gricourt, Vanessa Demontant, Elisabeth Trawinski, Melissa N’Debi, Camille Gomart, Guilhem Royer, Nathalie Launay, Jeanne-Marie Le Glaunec, Charlotte Wemmert, Giulia La Martire, Geoffrey Rossi, Raphaël Lepeule, Jean-Michel Pawlotsky, Christophe Rodriguez, Paul-Louis Woerther
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-04-01
Series:Frontiers in Microbiology
Subjects:
shotgun metagenomics
molecular diagnostic
pathogen identification
Sanger sequencing of the 16S rRNA gene
microbial documentation
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2022.761873/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2022.761873/full

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