Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications
Liver disease is one of the leading causes of death worldwide, leading to the death of approximately 2 million people per year. Current therapies include orthotopic liver transplantation, however, donor organ shortage remains a great challenge. In addition, the development of novel therapeutics has...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/7/2/36 |
| _version_ | 1827719066447511552 |
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| author | João P. Cotovio Tiago G. Fernandes |
| author_facet | João P. Cotovio Tiago G. Fernandes |
| author_sort | João P. Cotovio |
| collection | DOAJ |
| description | Liver disease is one of the leading causes of death worldwide, leading to the death of approximately 2 million people per year. Current therapies include orthotopic liver transplantation, however, donor organ shortage remains a great challenge. In addition, the development of novel therapeutics has been limited due to the lack of in vitro models that mimic in vivo liver physiology. Accordingly, hepatic cell lineages derived from human pluripotent stem cells (hPSCs) represent a promising cell source for liver cell therapy, disease modelling, and drug discovery. Moreover, the development of new culture systems bringing together the multiple liver-specific hepatic cell types triggered the development of hPSC-derived liver organoids. Therefore, these human liver-based platforms hold great potential for clinical applications. In this review, the production of the different hepatic cell lineages from hPSCs, including hepatocytes, as well as the emerging strategies to generate hPSC-derived liver organoids will be assessed, while current biomedical applications will be highlighted. |
| first_indexed | 2024-03-10T20:36:16Z |
| format | Article |
| id | doaj.art-d93a7224abf84477a37c7cb6f3f7899c |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T20:36:16Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-d93a7224abf84477a37c7cb6f3f7899c2023-11-19T21:04:21ZengMDPI AGBioengineering2306-53542020-04-01723610.3390/bioengineering7020036Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential ApplicationsJoão P. Cotovio0Tiago G. Fernandes1iBB-Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, PortugaliBB-Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, PortugalLiver disease is one of the leading causes of death worldwide, leading to the death of approximately 2 million people per year. Current therapies include orthotopic liver transplantation, however, donor organ shortage remains a great challenge. In addition, the development of novel therapeutics has been limited due to the lack of in vitro models that mimic in vivo liver physiology. Accordingly, hepatic cell lineages derived from human pluripotent stem cells (hPSCs) represent a promising cell source for liver cell therapy, disease modelling, and drug discovery. Moreover, the development of new culture systems bringing together the multiple liver-specific hepatic cell types triggered the development of hPSC-derived liver organoids. Therefore, these human liver-based platforms hold great potential for clinical applications. In this review, the production of the different hepatic cell lineages from hPSCs, including hepatocytes, as well as the emerging strategies to generate hPSC-derived liver organoids will be assessed, while current biomedical applications will be highlighted.https://www.mdpi.com/2306-5354/7/2/36human pluripotent stem cellshepatic cell lineageshepatocyte differentiationnon-parenchymal liver cellsliver organoidsdisease modeling |
| spellingShingle | João P. Cotovio Tiago G. Fernandes Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications Bioengineering human pluripotent stem cells hepatic cell lineages hepatocyte differentiation non-parenchymal liver cells liver organoids disease modeling |
| title | Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications |
| title_full | Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications |
| title_fullStr | Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications |
| title_full_unstemmed | Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications |
| title_short | Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications |
| title_sort | production of human pluripotent stem cell derived hepatic cell lineages and liver organoids current status and potential applications |
| topic | human pluripotent stem cells hepatic cell lineages hepatocyte differentiation non-parenchymal liver cells liver organoids disease modeling |
| url | https://www.mdpi.com/2306-5354/7/2/36 |
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