Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen
Abstract Background Zinc oxide nanoparticle (ZNP) has been applied in various biomedical fields. Here, we investigated the usage of ZNP as an antigen carrier for vaccine development by combining a high affinity peptide to ZNP. Results A novel zinc oxide-binding peptide (ZBP), FPYPGGDA, with high aff...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2016-11-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12951-016-0229-2 |
| _version_ | 1811302143408209920 |
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| author | Na-Young Ha Hyun Mu Shin Prashant Sharma Hyun Ah Cho Chan-Ki Min Hong-il Kim Nguyen Thi Hai Yen Jae-Seung Kang Ik-Sang Kim Myung-Sik Choi Young Keun Kim Nam-Hyuk Cho |
| author_facet | Na-Young Ha Hyun Mu Shin Prashant Sharma Hyun Ah Cho Chan-Ki Min Hong-il Kim Nguyen Thi Hai Yen Jae-Seung Kang Ik-Sang Kim Myung-Sik Choi Young Keun Kim Nam-Hyuk Cho |
| author_sort | Na-Young Ha |
| collection | DOAJ |
| description | Abstract Background Zinc oxide nanoparticle (ZNP) has been applied in various biomedical fields. Here, we investigated the usage of ZNP as an antigen carrier for vaccine development by combining a high affinity peptide to ZNP. Results A novel zinc oxide-binding peptide (ZBP), FPYPGGDA, with high affinity to ZNP (K a = 2.26 × 106 M−1) was isolated from a random peptide library and fused with a bacterial antigen, ScaA of Orientia tsutsugamushi, the causative agent of scrub typhus. The ZNP/ZBP-ScaA complex was efficiently phagocytosed by a dendritic cell line, DC2.4, in vitro and significantly enhanced anti-ScaA antibody responses in vivo compared to control groups. In addition, immunization with the ZNP/ZBP-ScaA complex promoted the generation of IFN-γ-secreting T cells in an antigen-dependent manner. Finally, we observed that ZNP/ZBP-ScaA immunization provided protective immunity against lethal challenge of O. tsutsugamushi, indicating that ZNP can be used as a potent adjuvant when complexed with ZBP-conjugated antigen. Conclusions ZNPs possess good adjuvant potential as a vaccine carrier when combined with an antigen having a high affinity to ZNP. When complexed with ZBP-ScaA antigen, ZNPs could induce strong antibody responses as well as protective immunity against lethal challenges of O. tsutsugamushi. Therefore, application of ZNPs combined with a specific soluble antigen could be a promising strategy as a novel vaccine carrier system. |
| first_indexed | 2024-04-13T07:22:50Z |
| format | Article |
| id | doaj.art-d947d68f74304041bf8c4a4d1ac2ff16 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-13T07:22:50Z |
| publishDate | 2016-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-d947d68f74304041bf8c4a4d1ac2ff162022-12-22T02:56:33ZengBMCJournal of Nanobiotechnology1477-31552016-11-0114111210.1186/s12951-016-0229-2Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigenNa-Young Ha0Hyun Mu Shin1Prashant Sharma2Hyun Ah Cho3Chan-Ki Min4Hong-il Kim5Nguyen Thi Hai Yen6Jae-Seung Kang7Ik-Sang Kim8Myung-Sik Choi9Young Keun Kim10Nam-Hyuk Cho11Department of Microbiology and Immunology, Seoul National University College of MedicineDepartment of Microbiology and Immunology, Seoul National University College of MedicineDepartment of Microbiology and Immunology, Seoul National University College of MedicineDepartment of Materials Science and Engineering, Korea UniversityDepartment of Microbiology and Immunology, Seoul National University College of MedicineDepartment of Microbiology and Immunology, Seoul National University College of MedicineDepartment of Microbiology and Immunology, Seoul National University College of MedicineDepartment of Microbiology, Inha University School of MedicineDepartment of Microbiology and Immunology, Seoul National University College of MedicineDepartment of Microbiology and Immunology, Seoul National University College of MedicineDepartment of Materials Science and Engineering, Korea UniversityDepartment of Microbiology and Immunology, Seoul National University College of MedicineAbstract Background Zinc oxide nanoparticle (ZNP) has been applied in various biomedical fields. Here, we investigated the usage of ZNP as an antigen carrier for vaccine development by combining a high affinity peptide to ZNP. Results A novel zinc oxide-binding peptide (ZBP), FPYPGGDA, with high affinity to ZNP (K a = 2.26 × 106 M−1) was isolated from a random peptide library and fused with a bacterial antigen, ScaA of Orientia tsutsugamushi, the causative agent of scrub typhus. The ZNP/ZBP-ScaA complex was efficiently phagocytosed by a dendritic cell line, DC2.4, in vitro and significantly enhanced anti-ScaA antibody responses in vivo compared to control groups. In addition, immunization with the ZNP/ZBP-ScaA complex promoted the generation of IFN-γ-secreting T cells in an antigen-dependent manner. Finally, we observed that ZNP/ZBP-ScaA immunization provided protective immunity against lethal challenge of O. tsutsugamushi, indicating that ZNP can be used as a potent adjuvant when complexed with ZBP-conjugated antigen. Conclusions ZNPs possess good adjuvant potential as a vaccine carrier when combined with an antigen having a high affinity to ZNP. When complexed with ZBP-ScaA antigen, ZNPs could induce strong antibody responses as well as protective immunity against lethal challenges of O. tsutsugamushi. Therefore, application of ZNPs combined with a specific soluble antigen could be a promising strategy as a novel vaccine carrier system.http://link.springer.com/article/10.1186/s12951-016-0229-2Zinc oxide nanoparticleZnO binding peptideScrub typhusVaccine |
| spellingShingle | Na-Young Ha Hyun Mu Shin Prashant Sharma Hyun Ah Cho Chan-Ki Min Hong-il Kim Nguyen Thi Hai Yen Jae-Seung Kang Ik-Sang Kim Myung-Sik Choi Young Keun Kim Nam-Hyuk Cho Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen Journal of Nanobiotechnology Zinc oxide nanoparticle ZnO binding peptide Scrub typhus Vaccine |
| title | Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen |
| title_full | Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen |
| title_fullStr | Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen |
| title_full_unstemmed | Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen |
| title_short | Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen |
| title_sort | generation of protective immunity against orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with scaa antigen |
| topic | Zinc oxide nanoparticle ZnO binding peptide Scrub typhus Vaccine |
| url | http://link.springer.com/article/10.1186/s12951-016-0229-2 |
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