Chip-based digital PCR for direct quantification dynamic bacterial load in target organs of tilapia infected with Streptococcus agalactiae, a pathogen causing meningoencephalitis in teleosts

To detection of pathogens DNA is considered a promising strategy in sick fish. The current standard for enumeration of pathogens to obtain colony forming units by plate counts has several drawbacks: long time to results, high variability. While qPCR can target genetic identity, its limitations requi...

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Bibliographic Details
Main Authors: Yuan Li, Bolin Fan, Dandan Li, Shun Zhang, Jichang Jian, Zaohe Wu, Yishan Lu, Zhongliang Wang, Wang Bei
Format: Article
Language:English
Published: Elsevier 2020-11-01
Series:Aquaculture Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2352513420306414