Yishen Qingli Heluo Granule Ameliorates Renal Dysfunction in 5/6 Nephrectomized Rats by Targeting Gut Microbiota and Intestinal Barrier Integrity
Chronic kidney disease (CKD) is often accompanied with imbalanced gut microbiota and impaired intestinal barrier. Hence, efforts to ameliorate renal dysfunction by manipulating gut microbial ecosystem are underway. Yishen Qingli Heluo granule (YQHG) is a representative traditional Chinese medicine (...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.858881/full |
| _version_ | 1811333480149155840 |
|---|---|
| author | Xian Sun Jie Chen Jie Chen Yiting Huang Sha Zhu Shuaishuai Wang Zijing Xu Junfeng Zhang Wei Sun |
| author_facet | Xian Sun Jie Chen Jie Chen Yiting Huang Sha Zhu Shuaishuai Wang Zijing Xu Junfeng Zhang Wei Sun |
| author_sort | Xian Sun |
| collection | DOAJ |
| description | Chronic kidney disease (CKD) is often accompanied with imbalanced gut microbiota and impaired intestinal barrier. Hence, efforts to ameliorate renal dysfunction by manipulating gut microbial ecosystem are underway. Yishen Qingli Heluo granule (YQHG) is a representative traditional Chinese medicine (TCM) prescription for clinical treatment of CKD. However, its underlying mechanism has not been well elucidated. This study aimed to explore effects of YQHG on renal dysfunction in 5/6 nephrectomized rats by targeting gut microbiota and intestinal barrier. Here, we found that YQHG provided significant renal protection in 5/6 nephrectomized rats by reducing renal fibrosis and inflammation, reestablishing bacterial communities, and improving intestinal barrier. Our analysis showed that YQHG altered the bacterial community of 5/6 nephrectomized rats. In particular, the prescription significantly increased the relative abundance of SCFA-producing bacteria (i.e., Lactobacillaceae, Lactobacillus and Lactobacillus_gasseri), which was contributed to the improved SCFA concentration (i.e., total SCFA, acetic acid, butyric acid) and intestinal barrier (i.e., the improved permeability and microbial translocation). More critically, microbiota-transfer study showed that the protective effect of YQHG was partly attributed to the mediation of the gut microbiota, especially the SCFA-producing bacteria. Our current findings propose a microbiota-targeted intervention and indicate that YQHG may become a novel promising treatment for CKD. |
| first_indexed | 2024-04-13T16:53:27Z |
| format | Article |
| id | doaj.art-d97db47366e247bfa436eeab78781ec4 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-13T16:53:27Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-d97db47366e247bfa436eeab78781ec42022-12-22T02:38:53ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-06-011310.3389/fphar.2022.858881858881Yishen Qingli Heluo Granule Ameliorates Renal Dysfunction in 5/6 Nephrectomized Rats by Targeting Gut Microbiota and Intestinal Barrier IntegrityXian Sun0Jie Chen1Jie Chen2Yiting Huang3Sha Zhu4Shuaishuai Wang5Zijing Xu6Junfeng Zhang7Wei Sun8The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, ChinaThe First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, ChinaHanlin College, Nanjing University of Chinese Medicine, Nanjing, ChinaThe First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, ChinaThe First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Medicine and Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Medicine and Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Medicine and Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaDepartment of Nephrology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, ChinaChronic kidney disease (CKD) is often accompanied with imbalanced gut microbiota and impaired intestinal barrier. Hence, efforts to ameliorate renal dysfunction by manipulating gut microbial ecosystem are underway. Yishen Qingli Heluo granule (YQHG) is a representative traditional Chinese medicine (TCM) prescription for clinical treatment of CKD. However, its underlying mechanism has not been well elucidated. This study aimed to explore effects of YQHG on renal dysfunction in 5/6 nephrectomized rats by targeting gut microbiota and intestinal barrier. Here, we found that YQHG provided significant renal protection in 5/6 nephrectomized rats by reducing renal fibrosis and inflammation, reestablishing bacterial communities, and improving intestinal barrier. Our analysis showed that YQHG altered the bacterial community of 5/6 nephrectomized rats. In particular, the prescription significantly increased the relative abundance of SCFA-producing bacteria (i.e., Lactobacillaceae, Lactobacillus and Lactobacillus_gasseri), which was contributed to the improved SCFA concentration (i.e., total SCFA, acetic acid, butyric acid) and intestinal barrier (i.e., the improved permeability and microbial translocation). More critically, microbiota-transfer study showed that the protective effect of YQHG was partly attributed to the mediation of the gut microbiota, especially the SCFA-producing bacteria. Our current findings propose a microbiota-targeted intervention and indicate that YQHG may become a novel promising treatment for CKD.https://www.frontiersin.org/articles/10.3389/fphar.2022.858881/fullchronic kidney diseaseyishen qingli heluo granulegut microbiotaintestinal barriermicrobial translocation |
| spellingShingle | Xian Sun Jie Chen Jie Chen Yiting Huang Sha Zhu Shuaishuai Wang Zijing Xu Junfeng Zhang Wei Sun Yishen Qingli Heluo Granule Ameliorates Renal Dysfunction in 5/6 Nephrectomized Rats by Targeting Gut Microbiota and Intestinal Barrier Integrity Frontiers in Pharmacology chronic kidney disease yishen qingli heluo granule gut microbiota intestinal barrier microbial translocation |
| title | Yishen Qingli Heluo Granule Ameliorates Renal Dysfunction in 5/6 Nephrectomized Rats by Targeting Gut Microbiota and Intestinal Barrier Integrity |
| title_full | Yishen Qingli Heluo Granule Ameliorates Renal Dysfunction in 5/6 Nephrectomized Rats by Targeting Gut Microbiota and Intestinal Barrier Integrity |
| title_fullStr | Yishen Qingli Heluo Granule Ameliorates Renal Dysfunction in 5/6 Nephrectomized Rats by Targeting Gut Microbiota and Intestinal Barrier Integrity |
| title_full_unstemmed | Yishen Qingli Heluo Granule Ameliorates Renal Dysfunction in 5/6 Nephrectomized Rats by Targeting Gut Microbiota and Intestinal Barrier Integrity |
| title_short | Yishen Qingli Heluo Granule Ameliorates Renal Dysfunction in 5/6 Nephrectomized Rats by Targeting Gut Microbiota and Intestinal Barrier Integrity |
| title_sort | yishen qingli heluo granule ameliorates renal dysfunction in 5 6 nephrectomized rats by targeting gut microbiota and intestinal barrier integrity |
| topic | chronic kidney disease yishen qingli heluo granule gut microbiota intestinal barrier microbial translocation |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.858881/full |
| work_keys_str_mv | AT xiansun yishenqingliheluogranuleamelioratesrenaldysfunctionin56nephrectomizedratsbytargetinggutmicrobiotaandintestinalbarrierintegrity AT jiechen yishenqingliheluogranuleamelioratesrenaldysfunctionin56nephrectomizedratsbytargetinggutmicrobiotaandintestinalbarrierintegrity AT jiechen yishenqingliheluogranuleamelioratesrenaldysfunctionin56nephrectomizedratsbytargetinggutmicrobiotaandintestinalbarrierintegrity AT yitinghuang yishenqingliheluogranuleamelioratesrenaldysfunctionin56nephrectomizedratsbytargetinggutmicrobiotaandintestinalbarrierintegrity AT shazhu yishenqingliheluogranuleamelioratesrenaldysfunctionin56nephrectomizedratsbytargetinggutmicrobiotaandintestinalbarrierintegrity AT shuaishuaiwang yishenqingliheluogranuleamelioratesrenaldysfunctionin56nephrectomizedratsbytargetinggutmicrobiotaandintestinalbarrierintegrity AT zijingxu yishenqingliheluogranuleamelioratesrenaldysfunctionin56nephrectomizedratsbytargetinggutmicrobiotaandintestinalbarrierintegrity AT junfengzhang yishenqingliheluogranuleamelioratesrenaldysfunctionin56nephrectomizedratsbytargetinggutmicrobiotaandintestinalbarrierintegrity AT weisun yishenqingliheluogranuleamelioratesrenaldysfunctionin56nephrectomizedratsbytargetinggutmicrobiotaandintestinalbarrierintegrity |