Enrichment and Quantification of Epitope-specific CD4+ T Lymphocytes using Ferromagnetic Iron-gold and Nickel Nanowires

Enrichment and Quantification of Epitope-specific CD4+ T Lymphocytes using Ferromagnetic Iron-gold and Nickel Nanowires

Abstract Epitope-specific CD4+ T lymphocytes were magnetically enriched using ferromagnetic Ni and Fe-Au nanowires coated with a monomer containing a major histocompatibility complex class II-bound peptide epitope (pMHCII). The enriched lymphocytes were subsequently quantified using fluorescence-act...

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Bibliographic Details
Main Authors: Daniel E. Shore, Thamotharampillai Dileepan, Jaime F. Modiano, Marc K. Jenkins, Bethanie J. H. Stadler
Format: Article
Language:English
Published: Nature Portfolio 2018-10-01
Series:Scientific Reports
Subjects:
Magnetic Enrichment
Paramagnetic Beads
Major Histocompatibility Complex Class II (MHCII)
Tetramer Solution
NiSi Nanowires
Online Access:https://doi.org/10.1038/s41598-018-33910-0

Internet

https://doi.org/10.1038/s41598-018-33910-0

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