Content of phenolic acids in rye caryopses determined using DAD-HPLC method
Phenolic compounds were extracted from rye caryopses with 80% (v/v) methanol. Phenolic acids were determined as free compounds and those liberated from soluble esters and glycosides. The analyses were performed using a Waters HPLC system equipped with a diode array detector (DAD). The following free...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2001-12-01
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| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-200106-0001_content-of-phenolic-acids-in-rye-caryopses-determined-using-dad-hplc-method.php |
| _version_ | 1797899911899906048 |
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| author | R. Amarowicz S. Weidner |
| author_facet | R. Amarowicz S. Weidner |
| author_sort | R. Amarowicz |
| collection | DOAJ |
| description | Phenolic compounds were extracted from rye caryopses with 80% (v/v) methanol. Phenolic acids were determined as free compounds and those liberated from soluble esters and glycosides. The analyses were performed using a Waters HPLC system equipped with a diode array detector (DAD). The following free phenolic acids were found: p-coumaric, ferulic and sinapic; the phenolic acids liberated from soluble esters were as follows: vanillic, caffeic, p-coumaric, ferulic and sinapic; and those liberated from soluble glycosides were the following: vanillic, p-coumaric, ferulic and sinapic. In rye caryopses, phenolic acids were chiefly in the form of soluble esters. A diode array detector was especially useful for the determination of vanillic acid: the UV spectrum of this compound showed a maximum at 260 nm whereas UV spectra of other phenolic acids were characterised by maxima at longer wavelengths. |
| first_indexed | 2024-04-10T08:37:28Z |
| format | Article |
| id | doaj.art-d99b3ecdcd35401683847a6e9e894744 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:37:28Z |
| publishDate | 2001-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-d99b3ecdcd35401683847a6e9e8947442023-02-23T03:26:31ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172001-12-0119620120510.17221/6608-CJFScjf-200106-0001Content of phenolic acids in rye caryopses determined using DAD-HPLC methodR. Amarowicz0S. Weidner1Institute of Animal Reproduction and Food Research Department of Food Science, Polish Academy of Sciences, OlsztynInstitute of Animal Reproduction and Food Research Department of Food Science, Polish Academy of Sciences, OlsztynPhenolic compounds were extracted from rye caryopses with 80% (v/v) methanol. Phenolic acids were determined as free compounds and those liberated from soluble esters and glycosides. The analyses were performed using a Waters HPLC system equipped with a diode array detector (DAD). The following free phenolic acids were found: p-coumaric, ferulic and sinapic; the phenolic acids liberated from soluble esters were as follows: vanillic, caffeic, p-coumaric, ferulic and sinapic; and those liberated from soluble glycosides were the following: vanillic, p-coumaric, ferulic and sinapic. In rye caryopses, phenolic acids were chiefly in the form of soluble esters. A diode array detector was especially useful for the determination of vanillic acid: the UV spectrum of this compound showed a maximum at 260 nm whereas UV spectra of other phenolic acids were characterised by maxima at longer wavelengths.https://cjfs.agriculturejournals.cz/artkey/cjf-200106-0001_content-of-phenolic-acids-in-rye-caryopses-determined-using-dad-hplc-method.phpphenolic acidsryehplcdiode array detector (dad) |
| spellingShingle | R. Amarowicz S. Weidner Content of phenolic acids in rye caryopses determined using DAD-HPLC method Czech Journal of Food Sciences phenolic acids rye hplc diode array detector (dad) |
| title | Content of phenolic acids in rye caryopses determined using DAD-HPLC method |
| title_full | Content of phenolic acids in rye caryopses determined using DAD-HPLC method |
| title_fullStr | Content of phenolic acids in rye caryopses determined using DAD-HPLC method |
| title_full_unstemmed | Content of phenolic acids in rye caryopses determined using DAD-HPLC method |
| title_short | Content of phenolic acids in rye caryopses determined using DAD-HPLC method |
| title_sort | content of phenolic acids in rye caryopses determined using dad hplc method |
| topic | phenolic acids rye hplc diode array detector (dad) |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-200106-0001_content-of-phenolic-acids-in-rye-caryopses-determined-using-dad-hplc-method.php |
| work_keys_str_mv | AT ramarowicz contentofphenolicacidsinryecaryopsesdeterminedusingdadhplcmethod AT sweidner contentofphenolicacidsinryecaryopsesdeterminedusingdadhplcmethod |