Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells

Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells

Summary: E-selectin is expressed on endothelial cells in response to inflammatory cytokines and mediates leukocyte rolling and extravasation. However, studies have been hampered by lack of experimental approaches to monitor expression in real time in living cells. Here, NanoLuc Binary Technology (Na...

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Bibliographic Details
Main Authors: Lydia Ogrodzinski, Simon Platt, Joelle Goulding, Cameron Alexander, Tracy D. Farr, Jeanette Woolard, Stephen J. Hill, Laura E. Kilpatrick
Format: Article
Language:English
Published: Elsevier 2023-07-01
Series:iScience
Subjects:
Molecular biology
Biotechnology
Cell biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004223013093

Internet

http://www.sciencedirect.com/science/article/pii/S2589004223013093

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